Stored sand still valuable? Should it be washed?


I took down my large tank a little over a year ago. I am about to start a 20 gal tank with rare Zoa's and leave most of my filtration to LR and and sand bed.

I have 5 gallons of sand in an inch of water that has been in a storage unit all this time. Do you think the cultures within the sand are still valuable? Should I was the sand? (I live in Texas so it has gone through 110F days)

Thank you