Zooxanthellae study

cpage3

New member
hey all,
i am currently involved in a laboratory at UB that studies zooxanthellae. The lab focuses on depth variation within the very diverse algae (broken down into clades as of now). The professor who runs the laboratory is Dr. Mary Alice Coffroth.

Anyways, I did an independent study during the semester and i am also going to work in her lab over the summer. I will be asking a question just like what was required in my independent study (i will try to post the paper summarizing my data soon). This time however i will be orienting my study around data obtained from captive corals.

Some possible questions to be asked include:

Are cladal distributions of coral variable with the intensity of lighting, or placement in the tank?

Do zoox clade associations correlate with that of wild counter parts, or does clade association change in a captive environment?

So i will be undertaking this study throughout the summer and have already begun extracting the DNA from corals in my tank.

In order for me to carry out this small study i will need a number of samplings of corals from many different tanks. A centimeter to centimeter and a half fragment is needed from a coral colony in order to perform experimentation (for hard corals), and 1-3 polyps of soft corals are needed from each colony (1 for small mushrooms and 3 for things like star polyps). Acropora and montipora are good candidates and are easy to harvest from the colonies. Soft corals are also needed. I am asking if local reefers would be able to help out in this endeavor.

If you are willing to donate a few small fragments of coral it will be greatly appreciated. Information that would be required for this study include:

Coral Genus and species (is not extremely important to have a 100% definite id)
Type of lighting
Size of tank
relative distance from light source
How long the coral has been in captivity
The area of collection (if known, not required)
the time coral has been captive in your system

I will be at the frag swap on the 20th of may at aquatech from 2:30 till about 3:30-4 and will try to leave storage containers for samples there after I leave. Samples will be immediately stored in ethanol or DMSO and you will be able to see the pigments in the coral leach out b4 your very eyes, and no this is not a scam to get free frags.

Special instructions are that you just bring them in tank water in order to keep them alive until put into the ethanol. This study is using similar techniques of the study posted in to coral forum (check it out for more information). Also, like i said I will try to post my write up on the data obtained in my independent study to give you an idea of what I will be doing. i think you guys will find it very interesting. i will also have two or three copies at the frag swap.

Interest in helping out in this way should be replied to on this thread. Thanks in advanced for your help and I hope to hear from you within the next few days.

Thank you very much
best regards
Christopher A. Page
 
Ok so I went to the lab today and I prepared 70, 1.5 ml tubes and filled them with 95% ethanol to preserve the samples.

In order to stream line the process please come to the meet with the frag's specs needed along with your name (listed above) on a sheet of loose leaf paper (this will make it much easier for me to coordinate).

I will reiterate how much tissue/skeleton is needed. It is a very unsubstantial amount but alot can be done with a small amount using molecular techniques. For sps a piece about as long as your thumb nail is all that is needed, it should be about the width of a single sps branch. For soft corals/lps an amount of tissue that is about as long as your pinky nail (when expanded) is all that is needed.

My goal is to be done with this study/question by the end of the summer. At the end of the study i will write up the data obtained and I will post it on this forum for you guys to look at. Again, in order for this experiment to be worth doing i need you guys. If there are over 70 samples, just bring them anyways, I will go to the lab and fill more tubes if i have to after.

Question number 1 (stated above) will be what I will focus on in my study

please respond to this thread if you are willing to donate samples.
Thank you very much
~CAP~



Please......please help out if you can 1 or two frags is fine it will definitely be appreciated. The goal here is to get as much variation in where these corals came from (in regards to setup) and tanks with all different kinds of lighting conditions are welcome.
 
Can we trade frags for the Ethanol?:p

I'm not going to be at the meet but I can drop frags by sometime if you'd like.
 
Hey Chris. What's the criteria for being considered a captive coral? In other words, how long in captivity before it qualifies for the experiment?

I have plenty of coral I can donate. I just need to know the cutoff time for being in captivity.

I can donate frags of both my A.efflorescens. Both have been in captivity since 2001, but under an assortment of different lighting over the last 5 years.

I have plenty of leathers to donate as well.
 
Let's set the criteria at at least 5 months in captivity. Any cladal changes in the algae probably will not be apparent until at least that long.
Thanks for your interest and pm me if you have any other questions.

Come on guys dont be shy help me out here

Thank you in advance
Chris
 
Please explain what the terms "clade" or "cladal" means. I've never heard those terms. What is the potential significance if cladal distributions are found to covary with lighting intensity? Inquiring minds want to know. Sounds like potentially interesting stuff. Good luck with your research.
 
Chris,
I could give ya a small Assortment of softie frags. Xenia,shrooms,a few different types of leathers etc... I won't be at the meet but live in Cheektowaga. I'm usually available at night after 6pm. Shoot me a PM if you wanna come by.

Brian
 
A trend in distribution of different clades (think of them as large groups of similar algae) as a function of depth/light intensity has been witnessed in various experiments throughout the carribean (in the pacific results can be sketchy). This experiment would try to determine if there is a similar, dissimilar, or no distribution according to light intensity (corals in the pacific show less structure as to what clade of algae a host coral harbors)

I posted a previous paper that I worked on in my independent study (sans figures, still workin on gettin them up) that will give you a little background and has some interesting results obtained though the samples are decidedly few in number. Take a look at it.

And as always I am still looking to fill some of the 70 specimen tubes I have lined up. I would appreciate it if you are able to help and will contribute. Im looking for as much variability in tank setup as i possibly can find.

Thanks again keep the posts coming
Chris
 
Let me reemphasize how much tissue is needed, NOT MUCH AT ALL, for instance a small clipping of a mushroom will suffice, or a nub from an sps colony. It is not my intention to gain new corals for my tank through this experiment, so donot bring extra for me (unless you want to), because anything left over i will try to grow from whats left (its a shame to waste in my opinion, but if you want me to throw out the extra just tell me, I wont take offense)

Thanks again
Chris
 

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