it won't kill your fish, it can kill bacteria and pods. I wouldn't run it 24/7 on a small tank, but something 250+ gallons it wouldn't cause a problem.
I wouldn't run it non-stop. But I'm not the best to ask as I've never used one. I don't see the point. If you do water changes and keep your water clean what's the use. But from what I've read and researched, they should be used less often than 24/7.
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