Could have been the bio pellets, I'm personally not a fan of bio pellets I see more problems with them then good. Not to say some aren't sucessful with them I would just go another route. I have been running my 90 gallon for 2.5 years with 17 fish and feed heavy and my nitrates are always around 10. I change water 10% a week and levels never seem to climb. I also do not run GFO because I feel it upsets the blance between phosphate and nitrate.