Fluorescence in green star polyps

[Mr31415]

First the polyp under normal transmitted illumination (10x)


20121116-DSLR_IMG_0012.jpg by pwnell, on Flickr
Green Star Polyps, 10x, POL+LP

Now for my hand at fluorescence.


20121228-DSLR_IMG_0017.jpg by pwnell, on Flickr
Green star polyp, 10x, GFP FLUO


20121228-DSLR_IMG_0015.jpg by pwnell, on Flickr
Green star polyp, 4x, GFP FLUO


20121228-DSLR_IMG_0024.jpg by pwnell, on Flickr
Green star polyp, 40x, GFP FLUO

 

[Michigan Mike]

What are you using an electron microscope?

 

[Mr31415]

No, an electron microscope does not work with light, hence cannot excite fluorophores in coral. This is a normal biological microscope.

 

[JaYbIrD1969]

Mr31415. Maybe you could start a thread that you document all of these awesome pics. I would love to subscribe to it.

Are you using actinic lighting to stimulate the flourescents?

 

[Mr31415]

Thanks for the comments. Actinic light - not really. I use a metal halide lamp through a GFP filter cube that excites the coral with blue light between the wavelengths of 446 – 486 nm.

 

[Mr31415]

duplicate.