nannochloropsis phytoplankton culture (questions)

[CLINTOS]

So far I started culturing nannochloropsis phytoplankton indoors using the petri dish method inwhich will be used to add directly to my saltwater tank that is at 1.024

I added a little less then 1/4 cup of rodi water that was boiled in the microwave for 1 min then I let it cool to room temp and added it to the petri dish then placed it under 2 normal output plant light's for 24 hours

where do I go from here?

I read that I should use the q-tip and add the entire dish to 1 liter of water under a gentle rolling boil and leave for 12-24 hours

my question is do I add this to fresh water or saltwater?

because ultimately I want the culture to be saltwater @ 1.020 ppm

and should I give the algae a break for a time period between adding the dish that was being lit for 24 hours to be lit in the 1L with flow for 16 hours?

I'll have more question's as I go

 

[sterling18]

I culture nannochloropsis in 1.020 sw.

I use Melev's method for culturing. Have been doing it for a couple of years. Good stuff.

You can add your starter to a bottle and provide light and air.

 

[rkelley_10]

2-liter bottle
--Saltwater mixed to 1.019 SG
--Air pump ----->> tubing through the cap push until it almost reaches the bottom
--PhytoGrow or some other aseptic fertilizer (don't use MiracleGrow)
--60W 6,500K CFL shop light
--Harvest in an average of 2 weeks. Conserve some of this culture to start the next batch
--Put the harvested phyto in the fridge and make sure to rotate the bottle at least once a day
--If you're particular, you can get a phytoplankton sieve to avoid adding culture water to your tank
--You can enrich live artemia
--You have arrived

That barely even made sense to me but that's what I do and it works. Hope it helps