Plankton experimental tank - some observations

ezrec

Member
Some observations from my experimental plankton tank.

-------- Background ------------

I have a 55g display + 15g system, with a vortex impeller circulation pump (600GPH w/two eductors) and a 100GPH sump pump with a vane impeller.

I have only 5lbs of live rock in the entire system, since I am studying a low-predation plankton system.

The substrate is egg crate on the glass, with a thin sand bed (1") of 50% crushed coral and 50% argonite sugar sand. The egg crate forms pockets of anerobic areas in the thin substrate.

The sump first-stage (heater and skimmer) has a 2" sand bed for additional nitrogen fixing.

Parameters (constant throughout the experiment):

S.G.: 1.024
pH: 8.4-8.3
Temp: 72F-82F
Amm/Nitate/Nitrite = 0 (occasional Ammonias spike when changing parameters, but that's it).

The refugium has a small amount of chaeto, but it grows very slowly due to the high quantity of phyto plankton which get first dibs on the nitrogen products.

All water is Pittsburgh City tap (tested to have < 0.1ppm Phosphorus, and no detectable Copper) with 1tsp/5gallons of "Start Right" dechlorinator.

My topoff rate is approx 0.75 gallons/day, varying by ambient humidity.
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Anyway, some of the observations:

* If you don't have very much live rock to act as a phytoplankton predatory system, your tank can stay pea-soup green for weeks, killing all green macro algae due to light starvation.
* Red macro-algae is unaffected by a phytoplankton bloom - in fact, it seems to grow pretty well under those conditions. Its chlorophyll appears to be able to utilize the green wavelengths that the phytoplankton does not absorb.
* Pea-soup green phytoplankton will absorb almost all blue light within 12 inches of water column.
* A Seaclone 150 skimmer makes almost no impact on plankton population.
* A impeller-based sump pump kills almost everything over 2mm in size, but does not impact anything under 1mm.
* Actinic-only lighting will reduce a phytoplankton bloom's opacity by 50% every week, but the opacity will rebound by 100% every day that full spectrum is used, until it reaches maximal opacity. Opacity does not equal population density - they just fade, they don't die.
* The fastest way to crash a phytoplankton system, and switch it over to a zooplankton system, is to add 1Tbsp/20 Gallons of dissolved table sugar.
* Full conversion from phyto-plankton dominated (green water) to zooplankton dominated (yellow water) took under 36 hours.
* A sugar-invoked zooplankton system will eat all of your calcerous red algae within four to five days. It's actually pretty amazing. Scary, but amazing.
* Chaeto does not grow at all, and in fact slowly dies, in a phytoplankton dominated system.
* Chaeto appears to double in size every 72 hours in my zooplankton dominated system.

I'm still waiting for my zooplankton system to crash (due to lack of phytoplankton and calcerous algae :( ), but I though you'd be interested in my current results.
 
the sugar made it a bacteria tank, not zooplankton :)


Also are you sure about the pH readings with the tank in a full phytoplankton bloom? (pea soup)
 
Yes, lots of bacteria, but as they are not photosynthetic, I count them in the zooplankton side of the equation.

And that which is opaquing the water is macro-scale: bacteria aren't dust-grain size and visible, nor do they and crawl over glass slides at 1mm/minute.

It's fun watching the juvenile flatworms snap them up, though.
 
<a href=showthread.php?s=&postid=10855208#post10855208 target=_blank>Originally posted</a> by ezrec
pH readings - they've been stable at 8.3 - 8.4 the whole time.

then your pea soup is also bacteria not phytoplankton
 
So green smells-like-grass soup that under microscopy consists of algal cells and eukariotes (flaggellaed cell with chloroplasts) don't count as phytoplankton these days?!
 
Are you talking thickness or opacity of pea-soup?

I'm talking "pea soup" color, and 1" to total opacity.

Now, the pea-soup thick sludge in my skimmer - that propably had a pH of 10, but I never tested my skimmer scum.
 
I'm talking opacity, it's well above 9 at far less then pea soup color in all my phyto cultures. your pH readings seam very off to me.
 
Do you run a single-species phytoplankton culture?

I think that may be the difference - I had a phytoplankton dominated setup, but with lots of other critters in the mix (ranging in size from bacteria to copepods) that would lower the pH with their CO2 production. I'm trying to emulate runaway tank scenarios, not culture a single phytoplankton species.
 
this is not zoo plankton you are culturing as stated by others, it sounds more like you are culturing algae and there copepods feeding off of it.
 
Maybe we're talking with different definitions here. Here's what I'm talking about:

* Phytoplankton - all life forms <1mm in size that (a) live fully suspended in the water column and (b) have chloroplasts
- Includes zoanthellae, diatoms, algae, etc.

* Zooplankton - all life forms <1mm in size that (a) live fully suspended in the water column and (b) do not have chloroplasts
- Includes bacteria, suspended copepods, worm larvae, etc.

Clear as mud?
 
Maybe we're talking with different definitions here. Here's what I'm talking about :

* Phytoplankton - all life forms <1mm in size that (a) live fully suspended in the water column and (b) have chloroplasts
- Includes zoanthellae, diatoms, algae, etc.

* Zooplankton - all life forms <1mm in size that (a) live fully suspended in the water column and (b) do not have chloroplasts
- Includes bacteria, suspended copepods, worm larvae, etc.

Clear as mud?

I just happen to be a stones throw from some one extremelly versed in this subject so I shot him over a quick email for his reply to your definition used above. My question to him was, isn't there a bactoplankton group and doesn't bacteria belong in that group? Here's his reply:

This is of course simplified for the sake of brevity:

First, plankton is all life that lives suspended in the water column; may be able to swim, such as flagellate algae and copepods, but unlike other organisms like fish, squid etc. not enough to move themselves out of the water mass they finds themselves in.

Phytoplankton (phyto- means plant) has historically meant all photosynthetic plankton, so includes all eukaryotic algae (including diatoms) plus the prokaryotic cyanobacteria (blue-green algae) and prochlorophytes (a group discovered only about 30 years ago). Zooxanthellae are not plankton when they live within corals and other animals (the zoo- in the name refers to their symbiotic association with the animals) but the do occur at least occasionally free in the water, and then are part of the phytoplankton.

Zooplankton (zoo- means animal) is the non-photosynthetic plankton, excluding bacteria. So includes protozoa (radiolarians, the hugely abundant foraminifera, ciliates, etc.), many invertebrate and fish larvae, copepods, and even many larger invertebrates like krill, larvaceans, pteropod molluscs, jellyfish, etc., etc., etc.

That leaves bacterioplankton as a separate category.

Remember these are categories, not necessarily related groups of organisms; “Nature mocks human categories.”

Dr. Henry
 
Since it switched over to a zooplankton (and bacterioplankton) system, my pH has gradually dropped down to 8.1, before rising back up and settling at 8.2

The zooplankton hasn't crashed yet, and I got some of the spec-sized critters (the most abundant visible-to-the-naked-eye) critters under the microscope.

They apepar to be some sort of segmented worm larvae - they look like a Dixie cup full of marbles with a segmented tentacle that sweeps for food and provides locomotion.

Haven't found any pictures online (yet) of this critter, but it could be anything - I'll keep researching.
 
Very good work. Don't expect to get much interest here, this is almost a berlin only forum. I would be very interested to see what would happen if you replaced your pumps with one like this:

Bellows Pump
 
That pump would probably help me reach a much higher survival rate of >2mm plankton!

Unfortunately, I'm saving my cash for other projects (ie, 100lbs of Live Rock from TBS this Christmas), so I won't be able to set up a bellows pump like that anytime soon.

As it is, I still have way too much plankton. The system is starting to re-establish a phytoplankton population, but is still zoo- and bacterio- plankton dominated at this time.

On a lighter note, once my pH finished dropping, and the zoo population stabilized, the plankton stopped stripping off my calcerious red algea. All my orange calcerious algea on a Fiji rock has been stripped clean, though. :^(

I would say that my orange tree sponge is dead, but some bizzare white tissue is starting to grow up through the previously dead spicules. The sponge is normally a bright orange, so I'm guessing it's either bacterial colonies, or another sponge on the rock is taking advantage of the free silica.

I really need to get my time-lapse camera set up.
 
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