Here is what went down, when i woke up friday the fish was still alive but died about 5 minutes later. I had no idea what to do and after freaking out and removing the fish i tested the water. Ammonia 0, Nitrite 1.0, Nitrate around 20, PH 8.2, Phosphate .25 or so. At this time I dosed prime according to the bottle for nitrite help and I went out and got lots of water in the event it came down to doing another WC but hoped my filtration would take care of it. At around noon I fed a half cube of brine just to see if the remaining fish would eat and they did. The fish were still breathing rapidly so i tested again and Nitrite had not changed.
At 7pm another test revealed no change in Nitrite so a 10 gallon was set up and the goldflake placed in it. I had about 22 gallons left and changed out the water in the 55 with it. All fish breathing improved and Nitrite was around .5 so I felt better. Next morning Nitrite was .1 and by the end of saturday undetectable. I wondered if it was the new brine shrimp cubes i fed thursday night and friday so i tested it.
This was a new cubed brine shrimp i have never used in all my years. The test went as follows
1 cup of RO/DI tested perfect
1 cube of brine shrimp
5 minutes thaw time then tested water used to thaw brine shrimp
Ammonia .6, Nitrite 0, Nitrate 0 but a big black bug looking thing was in the cube as well
My question is, is ammonia normal like this or not. Also is it possible something happened to my Nitrobacter that just couldt handle the nitrite that resulted from the ammonia in the food.