<a href=showthread.php?s=&postid=6585972#post6585972 target=_blank>Originally posted</a> by Alaskan Reefer
If you're using a HOB refugium, there isn't enough surface area for any amount of sand to do anything as far as denitrification -- it just turns into a waste dump that you have to replace without any real benefit. A thin layer on the bottom for the pods to live in or just no substrate at all. My HOB refugium is bare bottom and the pods do just fine living in the chaetomorpha. I could have more of them with a little sand, but for me it isn't worth the hassle. Besides, a healthy and well trimmed crop of chaetomorpha will remove enough ammonia, nitrite, nitrate, and phosphate that the sand shouldn't matter much. If it's a big enough fuge to accomplish some denitrification with sand, then yes 4"+ so you basically have a remote DSB. The funcationality also depends also on how much bioload you have and how big your skimmer is -- my skimmer is starving right now with a light bioload (4 fish in a 125) and a very thick crop of chaeto which is doing most of the work...
Pod propogation (natural food) and a place to grow macro is what you want out of the thing -- I wouldn't get too worried about the sand...