Like I said above I really dont think it was the tube anem, the first clown was the one that had alot of symptoms and was also the WC clown, this is where I think brook was introduced. the rest really didnt show symptoms but died pretty quick after intraducing them, about a month, the last 2 only lasted a week or so, and completly disapeared from the tank. I wonder if the other 3 fish I have will host the parasite w/ no adverse affects, cause then I will never be able to have a clown.