I set up a tank one time, and kinda rushed things. I had some frags that i needed to hold, no fish, also had chaeto, and DT's phytoplankton. I had to water running for a few days, threw everything in. I dosed phytoplankton. things came around just fine. The bioload was very low to begin with. And i think the chaeto and phytoplankton helped buffer any nitrogen cycle. I never did see a spike in the amonia or nitrate/nitrites. I kept anything that would rot, out of the tank. i never doesed any zooplankton (ie rotifers or frozen foods).