No, its not refrigerated. When I spoke with the guy he got into a little more depth than I expected, and even though I am going to school for microbiology, alot of it was a bit over my head. Basically, what I got out of it was that they set up an entire little microbial ecosystem in the bottle of live bacteria. It doesn't need to be refrigerated but the bacteria will die after a shelf life of 2 years (I think, if I remember correctly). And the bottle smells pretty wicked when its open. That's not really anything related to this, but I just thought I would warn anyone who is thinking about getting it. I was caught off guard