Experimental Aquarium Setup

btreyes

Member
Hi Everyone,

My name is Brandon Reyes and I am a graduate student in Dr. Forest Rohwer's coral reef microbial ecology lab at San Diego State University. I'm designing a large aquarium experimental system adaptive for a variety of coral experiments and provides a water source that has stable physical and biological components. The system is in the rough planning stage, but I need a lot of help from experienced aquarists with the overall ideas and what I can do to make the project work. I would appreciate any feedback/ideas about the aquarium setup that is explained below.
The current plan is to have five main components, each with specific functions. The overall goal is to provide water with uniform water quality parameters and microbial communities to experimental chambers over a period of time.

Component 1 will be a large aquarium (approximately 2,500 L) that collects and filters recycled water from coral experiments and provides a stable microbial community for those experiments. The filtration will consist of Chaetomorpha, a large skimmer, PO4 reactor, and a large waterway of liverock. Filtering the water before it passes through liverock will prevent any excess nutrients from drastically changing the liverock's microbial community and will inoculate the filtered water. After the water passes through the liverock, the temperature and alkalinity of the water will be adjusted with a chiller and calcium reactor in a final chamber. Finally, the water will be mixed before flowing into an external pump and travelling to component 2.

Component 2 will be a large (over 400 liters), shallow frag tank that grows corals for experiments and further inoculates the water with microbes. Most of the flow will be delivered from the pump directing water from the previous component. Any additional flow required will be delivered by powerheads. The light will be from overhead AI units or similar adjustable LED's. Most of the corals housed in this setup will probably be Acropora yongei, Montipora sp. and Favids. The water from component 2 will then travel into components 3 and 4.

Components 3 and 4 will be functionally identical to each other and used for different experimental treatments. Water coming into these units will travel to adjustable connections that will flow into either small aquaria, niskins (cylinder-like chambers), cups or whatever experimental compartments the experimental designer chooses for their experiment. Experimenters will also have the option of diverting flow downwards toward the treatment sumps to relieve excess water pressure. The outflows from the experimental treatments will be connected and flow downwards towards the sump for treatment. The sumps below these components will contain filter socks, skimmers, PO4 reactors, activated carbon and an additional compartment for a chiller and Ca reactor. The additional compartment for a chiller and Ca reactor will give the options of components 3 and 4 to function autonomously from components 1 and 2 in case the researcher decides to use antibiotics or something else that could contaminate components 1 and 2. The water will then be pulled into another external pump before flowing into two tandem UV sterilizers in order to eliminate microbes from the experimental treatments before flowing back into component 1.

Please tell me if the system makes sense and any feedback, suggestions and/or critiques would be very much appreciated. I'm also still struggling with how much flow we will need the external pumps to move.

Thank you very much for your help.

Here's a link to a picture of the schematic:

http://s1274.photobucket.com/user/btreyes/media/aquarium schematic_zpsa3bhrqe9.png.html?sort=3&o=0
-Brandon
 

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Something this well thought out has to work lol. The flow rates gonna be tough get a pump you can adjust and doesn't mind a lot of back pressure.
 
Thanks! Do you have any suggestions for pumps? I still have no idea how many GPH and number of pumps I should even be shooting for... Should I base it off the volume of the experimental components? Does is matter if Component One has too low of flow, but the other smaller components have just the right amount of flow based off of my pump choices? Where would you put pumps in the schematic?
 
I would keep it simple by having a single controllable (ie: DC) return pump from your main sump, feeding all the others by gravity. Calculate you total system volume and aim for a reasonable flow through your main filtration sump of somewhere in the neighbourhood of 5-10x total system volume per hour. If your total volume changes, depending on different experimental setups, the DC pump will allow you to adjust and maintain reasonable flow through your filtration sump.
Use powerheads in the various auxiliary tanks to fine tune flow requirements in those specific areas.
As far as specific pump choices go, that is largely going to depend on your budget.
 
Thanks for the advice on flow for the aquarium. I'm not sure if we will be able to gravity feed the other components though, as we are limited for space and the large aquaria will most likely need to be on the floor. Our budget is slightly limited, we have some grant money, but we can't afford anything super expensive. Do you have any suggestions for middle ground- good pumps? We're def going to go with an external DC pump and I'm thinking around 3-4K GPH.
 
We also need some feedback and critiques for our design for the first component. I have the incoming water going through a filter sock, followed by chaetomorpha, then a skimmer and PO4 reactor to remove excess nutrients. The water will then flow-through a series of liverock channels to further filter the water and reinoculate the water with reef microbes. The water will flow in sections underneath the baffles to get from one compartment to another in a zig zag fashion. After flowing through the liverock, the water will be temp controlled with a chiller and heater and also treated for alk and Ca via calcium reactor with an additional carbon reactor to polish the water before traveling to the frag tank reef component 2. The water will circulate in a final compartment for homogenization and testing before leaving for component 2.

We're not completely sure if the water flowing around the baffles will work and if our component layout would be optimal. Any advice/critiques would be thoroughly appreciated. Thank you.

<a href="http://s1274.photobucket.com/user/btreyes/media/Ocean_equipment_zpsvlij3hla.jpg.html" target="_blank"><img src="http://i1274.photobucket.com/albums/y438/btreyes/Ocean_equipment_zpsvlij3hla.jpg" border="0" alt=" photo Ocean_equipment_zpsvlij3hla.jpg"/></a>
 
<a href="http://s1274.photobucket.com/user/btreyes/media/flow_zpsdjv7rn0k.png.html" target="_blank"><img src="http://i1274.photobucket.com/albums/y438/btreyes/flow_zpsdjv7rn0k.png" border="0" alt=" photo flow_zpsdjv7rn0k.png"/></a>
 
in between chambers 3&4 instead of flowing on the bottom to avoid channeling you might move it to the top. but i guess with rocks or rubble in there it wont matter much? i like the design i wish i thought of that instead of the way i went with mine. great job and ill be following this one
 
Thank you Martok. By channeling do you mean the water will preferentially only flow through the bottom and not mix with the top layers above?
 
that was my initial thought but would greatly depend on the setting of the rock/filter media if set diagonally in the chamber it would have to flow up then down then up then down and out. no need to change just depends on media used and placement. do you mind if i use your design for a personal sump im building i think the complexity and the simplicity are in almost perfect balance
 
Haha, yeah, you can totally use my design, just know that we might be publishing the design in a scholarly journal article, but that shouldn't present any problems.
 
Does anyone have any suggestions on how to uv sterilize water without having a pressurized flow setup? Someone told me that it's possible to devote a portion of the sump to uv sterilization. Can anyone give me some ideas on how to sterilize the water without pressurizing it? Thanks!
 
you could always black out a portion of the sump and slide bulbs in form the side, but flow rate would be too high i think unless you restricted outflow rate to less than 100gph but that depends on what your trying to sterilize
 
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