baobao,
So how is your fish doing? I just re-read my thread and it should have read - "ââ"šÂ¬Ã‚¦fresh salt waterââ"šÂ¬Ã‚¦" - I'm hoping that you assumed this! As far as formalin dips/baths go, it is critical to ensure you aerate during the bath. As far as pulling the fish, it's a tough call. Duration time should be 30-60 min, depending on the fish, the disease you're treating for and how your fish reacts. With tropical marine fish, I would not extend the treatment beyond 30 minutes and a 10-20 minute bath can be effective.
When I'm using formalin, if I can get 10 minutes in then I would pull the fish any time after that if it was reacting negatively up to the 30-minute mark. Only rarely would I treat a fish longer. In your case, I probably would have pulled the fish after 15 minutes, as I would have watched it very closely after it started to react negatively at the 10-minute mark. If it looked like it was getting worse, then I would have pulled it.
The reaction of the fish depends on many factors: type and size of fish, the size of the treatment vessel, water temperature, pH and other parameters. Even lighting can be factor, as a dark vessel will keep most fish calmer during the bath. Because of this you need to stay with the fish and pull it if it gets too stressed. Knowing when the fish has reach its limit ultimately depends on using your best judgement and experience. If there are signs of stress, rapid swimming/jumping (to get out), loss of equilibrium, very slow or very fast ventilation - the fish needs to be put back into fresh sea water. If signs start to show up immediately, then it often indicates you have the dose wrong, or something else is wrong with the water chemistry (incorrectly matched pH for example). However, judgement and experience plays a big role. Often fish react wildly when it put into bath treatment container/vessels simply because the handling and change of environment freaks them out. After 5-10 minutes they usually settle down. If they don't you have to quickly assess the situation and remove them if they appear to be going down hill and getting worse (i.e., a progressive deterioration) - then the fish should be pulled.
Hope this helps.