Well, you WILl stress them outr, but how do reduce stress is what your looking for...... well, i use "Stress-Zyme" and it supposedly helps alot to reduce stress to everything, by not "chasing" the fish around, and just letting them swim into the net or box will not stress them out as much as a dearful chase for their lives, and making SURE that the temps, and all the parameters are the same in the tank that you are putting them into temp. until you can put them into the DT. like, use water right out from the tank if you can