How To Concentrate Greenwater?

[dpieroni]

hello to all.. Is there a way to concentrate greenwater?
I have 10 gallons of greenwater outside, and I was thinking a way to filter witha mesh or something and then puting in the fridge...
anyone who can help me?
thanks
diego

 

[firebirdbandit]

Basically you need a pure culture (no contaimanation), some fertilizer and a lower salinity.

 

[dpieroni]

forget but I don't understand..

forget but I don't understand..

But I think you do not understand me. i have 10 gallons of greenwater outside in my house.
It has a strong green color. I has 10 days and I think I cannot make any dense.
Is there a way to filter the algae cells? to concentrate it a store in a fridge?
I was thinking in filtering or somethig 5 gallons and use the other 5 gallons to star a new culture..
thanks
diego

 

[olin]

Filtering doesn't really do much - with those cell counts, filters clog so fast you'll go through hundreds of dollars in filters to get a few liters of concentrate. Commercial ops use a low gravity centrifuge or cream separator. For home use, putting a bottle of it in the fridge will reduce cell motility (if you're using motile cells). Once they've settled, pour off the supernatant (clear stuff) and keep the slurry at the bottom. If they are resistant to settling, you can add 50% v/vof freshwater to increase their relative specific gravity to help them drop.

 

[dpieroni]

thanks olin!

thanks olin!

i will try with a one liter bottle in the fridge.. i wil put images if I have results..
diego

 

[FMarini]

You may consider using a filter or filter paper or if you have access to a centrifuge or elutriation system. The filter or filter paper is easist, and yes you loose cell counts but you want to just add your collected GW to whatever. i think the centrifuges work best, but require---a centrifuge.
Gravity works pretty well, and as mentioned above a 1G sedimentation should concentrate most of your phytos

 

[GreshamH]

elutriation system

Is that like a "transdencial flow" (sp?) filter Frank?

 

[dpieroni]

greenwater water...

greenwater water...

Ok . thanks for all the replys..
taking abiut this, is is bad to add the water that is in the greenwater? I am thinking that this water can have phosphates and nitrates but if the greenwater took them to grow..
What is best to add to a reef? the greenwater filtered? or just add the greenwater?
of course if you add 5 liters of greenwater you are adding 5 times and with less nutrients than if I add 5 litres to my reef...
thanks to all
diego

 

[FMarini]

Gresham you mean "Tangential flow" filtration, althou I like your transcendental stuff.

Elutriators are centrifugal seperators that use allow large continous volume seperation w/ low Gs. They work real well at concentrating small particles

Most folks don't like adding the fertilizer containing water from the phytos to their tanks, only becuase its add fertilzer to your tank, but in small quanities I can't see it as a problem. However products like DTs are cleansed of their nutrient broths and placed in more tank friendly (and phyto friendly) transfer media
frank

 

[The Cardinal]

I filter my live phytoplancton through a mesh to avoid the high level of nutrients in the culture water. I use 1 micron for my nannochlropsis and 10 micron for my Isochrysis. In my view the amount of nutrient input can be substantial if you don´t filter it. It also means that you avoid adding copper etc from the fertilizer that might not have been taken up by the phytoplancton.