I tried hypo unsuccessfully twice on a ~425 gallon system. I was at 1.008/1.009sg for 6 weeks the first attempt and 8 weeks the second attempt. I don't have a sandbed, but did have 300lbs of rock in the system. Some of it was relatively new (200lbs) and 100lbs came from an established tank. I religiously changed 10% of the water every 3-4 days to stay ahead of any ammonia spikes. I think my failures may have been caused by:
1. Taking 10-12 days to get down to 1.008 vs. the two recommended days. I was constrained by my RODI output. I suspect taking that long could have allowed some of the parasite to adapt to those conditions and although all the fish looked good after the treatment, it took 4-6 weeks before it came back.
2. I never calibrated my refractometer at 1.008 s.g. It read consistently when I compared it to a friend's at normal salinity levels, but maybe there was drift at the lower levels? I don't know as I never compared it when at the lower levels.
After the second failure, I pulled all the fish, treated with cupramine and ran RODI water through the system for 4 weeks. Of the three surviving fish (foxface, desjardini and hippo tangs), I still have them in a separate 75gal tank. I added a QT'd achilles to that tank 6 weeks ago to act as a "canary in the coal mine." So far, all fish are ich free and it will be another six weeks before I return them to the display tank. The main system has had new fish (put through a thorough QT process) in it for the past 6 weeks, and they're all doing excellent.
Good luck!