Andrew, you've got the idea 
Just set up with sanitized SW and the f/2, set and forget for a week. The old KISS principle is best
Like Sunny, I don't worry about pH Well buffered SW and the usual culture routine will be sufficient 
BTW my culturing perspective is from maintaining phyto to feed to rots and pods to feed to larval fish...it was the fish that were the research subjects 
And yes, to give someone a start up culture it is that nice clean subculture you want to be giving away.
I just use a reasonably clean room away from my cultures for handling the subcultures and the initial 2L flask culture. At work, it was my office, at home my kitchen. stepping up from the 2l to the 5gal carboy is done right on the shelf of my culture set up...no point in lugging around full carboys since the that 2L flask is typically enough to get a strong enough start to overwhelm any likely contaminants.
Your culture set up looks good, though your right about the temp management troubles. Since your bubbling air into the cultures strongly (and the subcultures have the foam stoppers), you'll be fine with fans for good air exchange so long as what is coming in isn't too warm. An AC unit would be ideal for perfect temp control, but might not be needed pending room temps in your house.
Just set up with sanitized SW and the f/2, set and forget for a week. The old KISS principle is best
Like Sunny, I don't worry about pH
Well buffered SW and the usual culture routine will be sufficient BTW my culturing perspective is from maintaining phyto to feed to rots and pods to feed to larval fish...it was the fish that were the research subjects And yes, to give someone a start up culture it is that nice clean subculture you want to be giving away.
I just use a reasonably clean room away from my cultures for handling the subcultures and the initial 2L flask culture. At work, it was my office, at home my kitchen. stepping up from the 2l to the 5gal carboy is done right on the shelf of my culture set up...no point in lugging around full carboys since the that 2L flask is typically enough to get a strong enough start to overwhelm any likely contaminants.
Your culture set up looks good, though your right about the temp management troubles. Since your bubbling air into the cultures strongly (and the subcultures have the foam stoppers), you'll be fine with fans for good air exchange so long as what is coming in isn't too warm. An AC unit would be ideal for perfect temp control, but might not be needed pending room temps in your house.
