Mine are at about 30 right now cause I had to fix something with my skimmer so it wasn't running for a few days and I just added a new fish. Ideally it should be under 5 on a growing tank like mine. An established tank fully stocked with adults I think you'd be safe around 20-25. I run 1300 GPH through my sump which is filtered through a micron sock, then by my protein skimmer, and then sent either to the DT or to my fuge. My fuge, home to a big ball of Chaeto, then feeds to to the return area as well. I also do weekly 10% water changes with ro/di.