<a href=showthread.php?s=&postid=12404337#post12404337 target=_blank>Originally posted</a> by fishfanatic06
So, how do you know when your corals reach saturation?
You have to take appropriate physiological measurements to make a P/I curve (photosynthesis vs. irradiance). There are three proxies people typically use as a proxy for photosynthesis: 1) O2 production (classic and commonly used), 2) CO2 fixation (usually using 14C, though measuring change in TCO2 works in water), or 3) producing an Relative ETR (electron transport rate) vs. I curve by measuring effective quantum yield of photosystem II with a PAM fluorometer.
Using O2 production or CO2 fixation works well for determining physiological responses to light intensity, as well as for measuring net production. More and better information can be produced by combining O2 production and CO2 fixation within an experiment (this is a lot of work though). Using a PAM fluorometer works well for determining physiological responses, but isn’t so hot for calculating net photosynthesis. Hence, it would work for our interest here, but isn’t a good method for many other applications. PAM fluorometry is also so-so at resolving rates of photosynthesis in large structures like corals or higher plants (i.e., things other than microalgae) at higher light levels. It isn’t the magic bullet a lot of folks seem to think it is, but it is a very useful tool.
Since none of this is at all feasible for anyone outside a lab that has such equipment (a diving PAM starts at about $16,000

), it is necessary to work off best-guess approximations produced by other folks. You can get an idea of the neighborhood you’ll see saturating light intensity for a given coral adapted to those conditions. As linked above, Dana Riddle has written marvelous articles about the subject. Having said that, PAM fluorometry is, again, no magic bullet, and I think it is important to use another method (e.g., O2 production) before we get too comfortable with the numbers we produce. If I had one criticism of articles like the one linked above, it would be that the datasets are rather small. While I strongly agree with the general sentiments, I would like to see more done before we start talking about saturating and photoinhibiting intensities for any coral species, especially those that live over a range of depths.
Chris