The standard you would need 30 lb of sand and 30 lb of rock.... How much rock depends on what type of rock you buy. Some is more porous but 1-1.5 lb per gal is a good starting point.
This is a picture of a 30 gal cube I just started and it has a similar footprint as the bio cube. I've got 30 lb sand and 30 lb rock.
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