AEFW new info and photos

here is a photo but it does not really do it justice but you might get the idea
larvaemass.jpg
 
see how the line at the bottome of the bowl looks cloudy? that is because there is this huge swarming mass of millions of something moving very rapidly - I apparently now will have to go back to my office and get my microscope to see if I can get more details
 
few be seen by the photo!
with microscope you/he/she can be understood better
I am informed on the job of the worms turbellaria they begin to eat the fabric of the corals from the base and deposition of eggs.
but they crawls as nudibranco they don't swim
 
OK so I got the microscope and checked them out - they are really fast - wow! they are very small and kind of football shaped (american football that is!) with a red interior substance or spot on the rear end - there are just millions of them in one drop of water - they just zip across at supersonic speeds - these are definitely motile larvae of something - they came off this heavily infected piece of coral in a small bowl of water so that seems to narrow down the possibilties - I'm not sure if corals themselves have swimming gametes (I think they are just free-floating) - it seems most likely that these are the AEFW motile larvae - I wish I had a way to show photos of this to you guys from the microscope
 
so we could not really look at them closely as they were moving so fast - we put one drop of prazipro on them (granted this was into just in one drop of water on the slide) but it killed them instantly - I think many even ruptured - a few were still intact - they have the same little pigmented vacuoles inside them that the AEFW do - they also have cilia all over their body as opposed to a single flagellum
 
How long can these things live with no food? I think a possible answer for those who are thinking of treating a whole system is to does something (FWE etc) that might not kill them unless over-dosed, but stun them or effect them enough to not eat. If this can be sustained should'nt they starve themselves off? Instead of going for a quick kill, can we effect them enough to get them to stop eating?

-John-
 
I have been staring at this thing under 1000x magnification and there seem to be many small rectangular pieces wtih rounded edges that are moving around inside this larger organism - they seem to be moving about on their own - it is a stretch but are these possibly small flat worms inside the larger motile organism that simply get transported to a new site and then released? they do not seem to be some sort of organelles as they are very numerous and can be seen to be moving about on their own and flexing etc
 
mntl - several have tried treating with FWE once per week over several weeks and it does not work over time - they simply come back
 
I have been working on trying the praziquantel - the first thing I noticed was the viscosity of this stuff - it is like syrup -they say that it contains oxybispropanol as a solubulizing agent and this must be what it making it so thick - it was impossible to even draw up into a fine needled syringe - then when I was finally able to get it into the test water, it formed an oil slick on the surface - I tried to mix it around well. but am not sure if any of the med is even in the water - after one hour and 45 minutes at containers of 1, 2 and 3x recommeneded dosage, the worms seem unaffected - this may be because the med is not soluble in water (not sure if this the case - I'll have to look into that) and is not reaching the AEFW in sufficient concentrations due to this - if it is not readily soluble in water, we may have to try much higher doses to reach appreciable water concentrations
 
the bayer site states that 0.4 grams are soluble in water - given that I am not sure why then prazi pro has this what appears to be a non-soluble in water solubulizing agent? it seems that if the drug is more soluble in that than water, then it is going to remain in the solubulizing agent instead even when it is added to water - seems to make more sense to then possibly try the praziquantel from pill form and grind it up like we did with the interceptor - the problem is that this is then not going to be readily available to all people as it is prescription only - that is why I wanted to try the prazi pro as it is readily available - I did find a dog worm med online that is called drontal, but I do not know if it is Rx, and another problem is that it contains two other meds that I know nothing about, but are supposed to help fight dog worm infections
 
<a href=showthread.php?s=&postid=7540572#post7540572 target=_blank>Originally posted</a> by dolt
mntl - several have tried treating with FWE once per week over several weeks and it does not work over time - they simply come back

I think what mntl was implying here is that they cannot live without a food source. Many have posted a 5-7 day life span without food in the system (no telling how old they were when they started documenting, though). Now, I'm sure this varies, but if the corals are removed from the system for long enough (mine were out for 30 days, while the colonies were in treatment-it was no fun and a lot of work), when the food source dries up, so will the flatworms in that tank. I don't think ayone has had success with FWE. Levamisole seems to work, but can be tricky in terms of mortality rate (as I found out). I didn't see a single AEFW after the first treatment with Levamisole, and have not seen any since. It has been just under two months since I ended treatments, though.
 
I think it was stated in one of the AEFW threads (may have even been this one, I don't recall where I read it) that a minimum "dry" period (IE: no Acros in the tank) of 24 days is required to starve out the AEFWs. That comes from a combination of their lifespan and gestation period, both of which are not guaranteed (but are pretty commonly accepted as accurate, or accurate enough) :).

5 days til they die without food
14 days for the eggs to hatch

24 days = 5 days til the AEFWs die, if they lay eggs on their last day then 14 more days to hatch and 5 more for those to die.

It is more common to carry this out for a month (or at least 4 weeks). During the "dry" period the acros are all going through what gflat65 outlined in, again, one of the AEFW threads, as well as several other posters have shared similar regimes. It involves putting all of your acros into a QT system and treating them weekly with levamisole and inspecting for eggs, etc. As gflat65 stated, it is no fun and generally alot of work all around.

HTH :)
 
sorry - maybe I misunderstood what mntl was saying - now that I re-read it I think he is talking about treating them in tank at lower med doses and leaving it in so that they are stunned enough to not eat but not die and they will eventually starve without eating
one interesting side note is that in one research paper I read online was that in some species there are two types of eggs - one that lasts for short periods laid in good conditions and another for when conditions are bad that can last for several months - sorry I don't have the link but I have read so much in the past few days and I have no idea where I found it
 
Wow! Flagellated mobile larvae? It would be nice if we had a marine biologist chime in here. I would love to hear there input. This thread has some good basic science in it.
 
yeah, but unfortunately no good success stories - the prazi pro at 5x recommended doses for about five hours did not seem to touch the AEFW - I am going to try some higher doses in a bowl but I would have more concerns about it's safety in the tank at such high doses
 
maybe I misunderstood what mntl was saying - now that I re-read it I think he is talking about treating them in tank at lower med doses and leaving it in so that they are stunned enough to not eat but not die and they will eventually starve without eating

This is exactly what I was saying. This seems like the least obtrusive thing to due for those who cannot remove the acros and must treat the display as it should require lower doses than one that kills them within hours. How much lavamisole (anything) etc. does it take to visibly make them react (squirm etc.), this should show that they are discomforted and possibly to enough of a point where they will not eat. Keep them in this state, which should kill them off in a week or so (starve them off) and then the eggs when they hatch. But atleast they would not be harming anything at this point.

This will definately take some experimenting though. Can anyone seclude them and try different products to see the minimum doseage it takes to make them react, even iff only slightly?

Sorry if I was unclear the first time through (or this time for that matter :) )

-John-
 
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