Kathy!
I was so thrilled by your article that copied it to another thread where people had unanswered questions about brine.
One member, RayJay, has replied to your comments, so I thought you have to know!!
The thread is at
http://reefcentral.com/forums/showthread.php?s=&postid=7706432#post7706432
Here is a copy of his reply as well (in case you can't open the thread) THANK YOU AGAIN!!!!
You can hatch out the brine at just about any s.g. you wish between 1.010 and up even above 1.040. They will hatch out faster at the lower s.g. though.
I only have one dissagreement with Kathy's statement and that's regarding the erronious part about live adult brine nutrition.
Great salt lake brine shrimp produced in grow out systems for aquaculture have a protein level running between 49% and 62% and wild brine from GSL have levels just a little higher.
Theses levels can be bettered by gut loading the brine shrimp.
Newborn nauplii are low in protein but rich in the fatty acids, but quickly loose the high levels of huffa content while protein levels increase for juveniles and adults.
If you are looking to feed the fatty acids, it's best to feed them to the target right after hatchout which varies with temperature, salinity and whether or not they have been decapsulated.
The figures for protein level come from the United Nations article on Live Foods for the Aquaculture Industry, edited by scientists from the Artemia Research Center at the University of Ghent. It is in section 4.4.1
This article is the most complete article I've ever found on Brine Shrimp and is part of a larger article including rotifers, phyto and other live foods.
CLICK HERE AND SCROLL DOWN TO SECTION 4.0
While brine shrimp can live at s.g. from a bit below 1.010 up to almost saturated solutions, I found best results in my grow out operations to be at normal salt water levels in the 1.025, at temperatures of 80F.
Because brine shrimp eat small particles in suspension in the water, its best to use phytoplankton where possible, with cryopastes second best. However, they will eat pretty well anything that is appropriately sized.
Grow out of brine shrimp can be easy, for low density cultures, and progressively harder and more labour intensive as the culture density increases.
It's best if you can buy them locally already grown out, remembering that they probably haven't been fed properly at the LFS so Gut load them before feeding to the fish.
Anyone interested in reading about my methods can go to my brine shrimp page:
RAISING BRINE SHRIMP TO ADULT
FWIW, I believe the nutrition misconception arose from the fact frozen brine shrimp usually have protein contents on the packages listed as a percentage of "wet" weight, including packaging fluids.
Mysis and other foods have protein contents listed as a percentage of "dry" weight. No fluids.
Mysis still have a slight edge when both are compared in "dry" weight percentages, but mysis are not as easy to grow and don't propagagate anywhere near as fast, so economically for the aquaculture industry the brine shrimp work out much better.
quote:
--------------------------------------------------------------------------------
This last article teaches you how to breed them to adulthood, so that they start having eggs themselves
--------------------------------------------------------------------------------
For cysts (eggs) to be produced, conditions have to be worsening as in increasing salinity.
Under normal conditions, the brine will produce live born nauplii.