I thought I'd share my findings from some research I've been doing on using Chaeto to export nutrients in my 10 gallon system.
Lighting: Photosaturation occurs at 400PAR [1]. I'm unsure of how this translates directly to PUR, but BRS talked about it in their review [2].
Photoperiod: I'm not aware of any studies on what ideal is. [1] states they're using 15:9 light:dark but then it appears to be the reverse on their charts. You can definitely see nutrients being reduced more quickly when the lights are on, but a resting period may be necessary. A common method is to use 12 hours on, 12 hours off.
pH: Increases when the algae is lit due to oxygen production and other factors [2].
Nutrient Export: A simple rule of thumb is 10g of dry macroalgae will reduce nitrate by 10ppm and phosphate by .1ppm in a 67-gallon aquarium [4]. For Chaeto, this varies based on the nutrient content of the water [1]. See the charts in [1] for estimates based on nutrient content and aquarium size.
Nutrient Limitation/Imbalance: Growth is P limited when N ratios exceed 11-24. Growth is N limited when N ratios drop below 8-16 [1]. When N is enriched, the algae can apparently transfer some of this for storage in the chlorophyll. Therefore, you may be able to determine nutrient imbalance just by looking at the color of the algae. "N deficiency typically decreases photosynthetic pigments (Falkowski and Owens, 1980)". Iron depletion may require dosing as well, although I'm unsure of symptoms other than slowing growth [5]
With this data, you should be able to estimate the amount of Chaeto needed to export a desired amount of nutrients from your systems and in what concentrations. Hopefully it helps many of you run more efficient 'fuges and solve issues with poorly growing macro.
[1] MARGARITA MENÉNDEZ1 and F.A. COMÃN1. Spring and Summer Proliferation of Floating Macroalgae in a Mediterranean Coastal Lagoon.
https://www.researchgate.net/public...tal_Lagoon_Tancada_Lagoon_Ebro_Delta_NE_Spain
[2] https://www.bulkreefsupply.com/vide...-best-refugium-light-and-what-is-coming-next/
[3] MARGARITA MENÉNDEZ1, JORGE HERRERA2 and F.A. COMÃN1. Effect of nitrogen and phosphorus supply on growth,
chlorophyll content and tissue composition of the
macroalga. http://scimar.icm.csic.es/scimar/pdf/66/sm66n4355.pdf
[4] http://reefkeeping.com/issues/2006-09/rhf/index.php
[5] http://www.advancedaquarist.com/2002/8/chemistry
Lighting: Photosaturation occurs at 400PAR [1]. I'm unsure of how this translates directly to PUR, but BRS talked about it in their review [2].
Photoperiod: I'm not aware of any studies on what ideal is. [1] states they're using 15:9 light:dark but then it appears to be the reverse on their charts. You can definitely see nutrients being reduced more quickly when the lights are on, but a resting period may be necessary. A common method is to use 12 hours on, 12 hours off.
pH: Increases when the algae is lit due to oxygen production and other factors [2].
Nutrient Export: A simple rule of thumb is 10g of dry macroalgae will reduce nitrate by 10ppm and phosphate by .1ppm in a 67-gallon aquarium [4]. For Chaeto, this varies based on the nutrient content of the water [1]. See the charts in [1] for estimates based on nutrient content and aquarium size.
Nutrient Limitation/Imbalance: Growth is P limited when N ratios exceed 11-24. Growth is N limited when N ratios drop below 8-16 [1]. When N is enriched, the algae can apparently transfer some of this for storage in the chlorophyll. Therefore, you may be able to determine nutrient imbalance just by looking at the color of the algae. "N deficiency typically decreases photosynthetic pigments (Falkowski and Owens, 1980)". Iron depletion may require dosing as well, although I'm unsure of symptoms other than slowing growth [5]
With this data, you should be able to estimate the amount of Chaeto needed to export a desired amount of nutrients from your systems and in what concentrations. Hopefully it helps many of you run more efficient 'fuges and solve issues with poorly growing macro.
[1] MARGARITA MENÉNDEZ1 and F.A. COMÃN1. Spring and Summer Proliferation of Floating Macroalgae in a Mediterranean Coastal Lagoon.
https://www.researchgate.net/public...tal_Lagoon_Tancada_Lagoon_Ebro_Delta_NE_Spain
[2] https://www.bulkreefsupply.com/vide...-best-refugium-light-and-what-is-coming-next/
[3] MARGARITA MENÉNDEZ1, JORGE HERRERA2 and F.A. COMÃN1. Effect of nitrogen and phosphorus supply on growth,
chlorophyll content and tissue composition of the
macroalga. http://scimar.icm.csic.es/scimar/pdf/66/sm66n4355.pdf
[4] http://reefkeeping.com/issues/2006-09/rhf/index.php
[5] http://www.advancedaquarist.com/2002/8/chemistry