Continuous live plankton feeding

joanxavier--

That is a really cool setup you have. I'll give you another :thumbsup:

Did you make that (it wasn't quite clear to me from the translation)? From what I can tell, on the upstroke of the machine, the syringes fill themselves through vacuum. On the downstroke, the syringes empty to your culture. How long does it take for the machine to cycle?

Do you use small check valves (one-way valves) on the tubing?

Thanks! Sorry about the awkwardness of the translation.
 
Andy, I´ll try in english: I think i´m getting hard work for you.
Sorry for my horrible english.
Yes, I did this doser myself, it´s really easy to do.
The doser gives about 10 ml per minute, so it takes about 20 hours running.
Ít´s very important feeding the culture slowly, this kind of feeding keeps the water quality and provides exact microalgae concentration in your culture.
I know your instan algae, I d´ont like to much because the algae mixed with liquid needs antioxidation compounds and the algae loose some propierties.
Also the rate price-concentration is not good.
I pay 20 â"šÂ¬ for 15 grs (dry wheigth) but Instant algae cost is 40 USD per 9 grs (dry wheigth). But the really important in this mathers are the propierties of algae. Freeze-dried algae d´ont loose any propiertie compare whith alive algae.
 
joanxavier,

If you think your English is "horrible", you have not experienced my Spanish!

Please continue to contribute here - the submissions are very worthwhile and much apprecialted!

Regards,
Bob D.
 
If you think your English is "horrible", you have not experienced my Spanish!

Please continue to contribute here - the submissions are very worthwhile and much apprecialted!

Thank you, radiata. Those are my feelings exactly!


Freeze-dried algae d´ont loose any propiertie compare whith alive algae.

Except, being dead, they immediately start to degrade the water. On the other hand, I'm experimenting with Clor-Am-X. Apparently, it can greatly increase the yield of rotifers and copepods by binding up free ammonia. But, point well taken on the cost.

I hope you don't mind, joanxavier, if I borrow from design. It is quite beautiful to my eye.
 
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Except, being dead, they immediately start to degrade the water.
Andy, there is not exact. I test many times the water culture, and never detect any ammonia or nitrites. The rotifers are growing very well with this algae sistem, and rotifers are very sensible to bad quality water. If you have a microscope, check how many eggs the famele has, and how many males are in this culture sistem. You can understand very well, what is happening about quality water. It´s impossible to obtain 700-900 rotifers/ ml. iff the water is not perfect one.
Iff freeze-dried algae is avalable for you, try to start a culture with this, and compare.
I can send to you a culture protocole for rotifers.

I hope you don't mind, joanxavier, if I borrow from design. It is quite beautiful to my eye
Never mind, it´s a pleasure for me
Thanks for your kidness with my spanglish:mixed
 
Please don't get me wrong.... I think by dosing so slowly and constantly you are giving the rotifers ample time to utilize the phytoplankton completely. Since you culture constantly, how do you keep the nitrates from eventually getting crazy? Do you incorporate de-nitrification methodologies, or do you clean and re-start your cultures occasionally?

I would like to see your culture protocol, but I don't want to ask too much work of you. What you've shown me already will certainly get me going.

Thanks!
 
This is my method culture.
I´m using this receptacles for rotifer culture
aire-sifon.jpg

This receptacles volume is about 4,5 liters.
The method consits in 2 receptacles
I call first receptacle "stump" and second receptacle "production"

The cycle takes 5 days from stump to production.
Stump growing...
1 day...inoculation stump 1000 ml. 800 rotifer/ml. from old culture and add 500 ml. green water ( 200 mg algae + 750 ml. water 1022 specific gravity) Total culture 1500 ml.

2 day...add 600 ml green water (200 mg algae + 750 ml. water 1022) Total culture 2100 ml

3 day...add 600 ml green water (300 mg. algae + 750 ml. water 1022) Total culture 2700 ml.

4 day...add 650 ml green water (300 mg. algae + 750 ml. water 1022) Total culture 3350 ml

5 day... add 650 ml. green water (400 mg. algae + 750 ml. water 1022). Total culture 4000 ml. Final density 700-900 rotifers/ml.
Culture look like this
transparencia.jpg


macroroti.jpg

densidad.jpg


rotisharko.jpg


6 day... collect 1000 ml old stump, now production, and start new stump like first day protocole. Add 1000 ml. green water (400 mg. algae + 1000 ml. water 1022) Total culture 4000 ml production receptacle + 1500 ml. stump receptacle

7 day...collect 1000 ml from production receptacle.Filter rotífers with 50 microns and feeding acuarium.
Production receptacle...continue like 6 day
Stump receptacle...continue like 2 day

Continue till new stump pass to production. When new stump pass to production, use old production culture for masive feeding acuarium (5 days feedeng acuarium with rotifers from 1000 ml. and 1 day masive feeding acuarium from 4000 ml.

With this method you dont need clean receptacles, just use clean receptacle when you start new stump. Any denitrification system is not necessary because is allways clean.

I´m afraid about my explanation, please excuse me
 
Sorry I didn't reply yesterday. You have a beautiful culture setup and that's one dense rotifer culture! May I ask a question? It looks like you are lighting your rotifer culture and I wonder why? Especially if you are using dried phyto.

Thank you again for being so generous with your time and photos.
 
Andy, I´m not lighting the culture.
This light is just for water transparence control and estimative rotifer concentration. I don´t acount rotifers by microscope every time, usualy I do it once a month. With experience is possible evaluate the concentration just looking the culture.
 
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