some will, some won't.
it's not really a one shot deal, which is why many people culture them outside of the tank environment in a separate vessel, then every so often harvest some to place in the tank.
just dumping one bottle of pods in the tank on time isn't going to do a whole lot of good.
setting up a fuge will help them have a good place to breed.
the best would be an external culture, and a fuge.