Culturing your own Phytoplankton

Sarah,

So you think in the gelatin idea that I should store them at room temp with lights? (I was thinking perhaps the freezer....Walt Disney style :P ). I guess I will have to do some tests once Im up and running.
 
DSB - Lemme try keeping some plates out this week and next at room temp and just adjacent to some of the timer-operated lights I have on my tank at home.. and I'll report back. I know that you can fridge them for a bit without them going bad.. so I'll have to try with the gelatin idea and see how long you can really push it. :) I was thinking of growing them a week and then fridging a week in a dark container so they dont get any light when I go to snack on something. That should mimic a vacation decently. I'll let you know - if you try it too we'll compare notes.

>Sarah
 
and more info from DT:

---------------

EPA is Eicosapentaenoic acid one of the most essential omega 3 fatty acids.

DHA is Docosahexaenoic acid, another essential omega 3 fatty acid.

Although animals can synthesize one from the other it is not as efficient and the goal from our continued research is to find a species of microalgae that would be a good source of DHA that survives refrigerated storage.

Note: Isochrysis and Pavlova are dead within 2 weeks of refrigerated storage.

People are being mislead by claims that they survive in
refrigerated storage.
 
So one would need to replace their DT's 2 weeks after they go in the fridge. Does DT date the bottles as he refrigerates them before shipping, and being stored in the fridge at the LFS? Two weeks total, and then the bottle we buy loses 2 species!

My nannochloropsis survived quite well in my fridge, and began to grow again right away. I think I had them in there a month. Nanno may be different this way than Iso and Pavlo.

It's quite clear to me that DT's is a superior product, and one would need to pay for the expertise, skill, and research it took to get this superior product,

unless one does not need such finesse.
 
Here's an overview. Nannochlorpsis oculata holds EDT best at 20 degrees and salinty 25-30. Dt's refridgegerates vitamin B12 and adds it separtly.
Can you find out anymore about there washing process? Do they make there own furtilzer from strach? How much does it cost to analize phytoplanton? Are there physical difference's between strains of the same phytoplanton or is chemical analyisis for the most part called for?

Great information I only wish I had more to share.
 
A 'lil bit more info...

I will also follow up with Mr Tagrin for more info he can/will share relating to new questions. For now:

--------------------
(response to Andy's concern/question)
"DT's does not contain Isochrysis or Pavlova because they do not survive storage. DT's has been proven to maintain good nutritional value through 5-6 months and to stay alive for many months beyond. (Toonen 2002). The shelf life is based on the feeding trials published in the 2002 Marine Fish & Reef Annual. DT's must be maintained in refrigerated storage and will be highly nutritious for 5 months from harvest. Dennis"
 
No doubt that DT's has the science and technology to provide a very high quality product but.......if you spent about 10 minutes a day you too can grow a great phytoplankton like I've been doing for the past couple of years. I'll be the first to admit that my phytoplankton isn't as good as DT's but then it doesn't cost me an arm and a leg to feed my tank.

As far as contamination goes, IME as long as you don't over fertilize (which will kill your culture anyway) you should be fine. IME it is extremely difficult to poison your tank by overfeeding phyto. for experiemental purposes I've dumped a 2 litre bottle of phyto into my 29 gal and notice no changes in any params. I did have many filter feeding organisms in the tank (no sps) to consume it though.

I agree I would first purchase and read thoroughly the phytoplankton culture manual by Flordia aqua farms. before I began a home culture.

Also, if you want to deal with serious science and phytoplankton check out the university of texas phytoplankton dept.
here's a link to their site and many others.

http://www.bio.utexas.edu/research/utex/index.html

and others

http://www.botany.utoronto.ca/utcc/Links.html

Home culture is relatively easy and inexpensive, figure it this way (and I'm not knocking DT's at all, its a awesome product). for about 3 - 4 16oz bottles of DT's you can grow your own phytoplankton, probably more than one species, and feed your tank as often as you like, and give away extra to fellow reefers.
How can you go wrong?

One last thing, I wonder if Dennis can really explain the smell of DT's?

does anyone here who has grown their own phyto have that terrible smell to it? Mine doesn't even when it goes bad.
 
What does it smell like? If it is sulphurous, it may be that sodium thiosulphate or some other sulphur compound is added to deactivate ammonia or chlorine.
 
it is a sulfurous smell but from what I figure phyto consume ammonia and so that would rule that out. Chlorine would kill phyto.
 
No doubt that DT's has the science and technology to provide a very high quality product but.......if you spent about 10 minutes a day you too can grow a great phytoplankton like I've been doing for the past couple of years.

AMEN!!
 
Or maybe us Michiganders are just a bunch of phyto loving fools, huh ediaz. I'll be out your way this weekend, Saugatuck
 
In reply about the smell: Yep. You right!

With all the talk about trace metals and fertilizer being dosed with the home grown phyto, I wonder if people are taking into consideration that the phyto consume these things, and that it doesn't dissappear. Even if you feed washed phyto to your tank, your phyto eating organisms are getting a dose of whatever the phyto consumed, and they consumed f/2 or whatever the phyto was grown on. So the trace contaminants in the water the phyto is grown in, is a small concentration when diluted in the tank water, compared to the amount the phyto eating corals and inverts are getting from the phyto itself. Who knows if this is not a required nutrient for them anyway?

I hang out with pharmacologists, and the expression goes, "Dose is everything."

Cheers,
Kathy
 
So it has been almost a week. My cultures look great (an opaque neon green) but not the intense dark green I have seen in other cultures. How long does it ussually take to reach that point? Also how can you tell if your culture crashes?

PS: My rotifer cysts came today, cant wait to set them up!!!

PPS: Can we sticky this thread for future culturists? (search is not my friend on this site :( )
 
It takes a while for the algae to get used to your water. Keep it clean to avoid crashes.

Get a loupe to see the rotifers when they hatch, the first time its hard to spot them.

if the culture crash, most times it will turn like a dirty yellow, it will foam a little and you will see sediment on the bottom of the culture container.

There is a sticky at The Fish Breeding Forum, with articles on live food cultures by Dr. Frank Marini, a must read.

Ed
 
Exceptional thread. It should be stickied.

Anthony. Thank you for taking the time to ask questions on our behalf and reporting back here. I must say it was very generous of Mr. Tagrin to share his knowlege with us.

I was dismayed to learn that phyto looses its DHA/EPA content above certain temps. I have no way to keep my phyto cool. We have been above 30 C for the whole summer.

It will be interesting to see how the temp affects seahorse fry survival rates.

Fred
 
just a note, don't waste your money buying a florida aqua farms or any other brand of plastic mearuring stick. Their pretty much useless. No accuracy whatsoever.
 
ezhoops said:
just a note, don't waste your money buying a florida aqua farms or any other brand of plastic mearuring stick. Their pretty much useless. No accuracy whatsoever.

No kidding?I find it very useful.Much more accurate than a visual estimate,and much simpler than a cell count.
 
I've noticed alot of algea growth in my display when adding home cultured phyto (but) I only added on one time so my system probally coulden't handle it.
 
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