i used as little sand in my 90 gal as possible. just enough to cover the bottom, like prob 1/2 inch. you dont want dangerous gases and nitrates to get trapped in your sand. also i clean my sand on every water change.
I have a DSB, there is evidence that it could release dangerous gases as Jr said, so do it at your own risk. I have had mine for about 2 years no prob. I even have a remote DSB as well. IMO, shallow sand beds look nicer.
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