Right on, same here.
How are things going? Sorry to hear you've had some losses. Are you back on track? I wonder what's happened. Maybe a delayed reaction to the new substrate addition? That seems unlikely, since it basically just adds filtration capacity. I've heard of mini-cycles happening in similar situations. Maybe there was a Nitrite spike.
Water changes seem like a good strategy. Changing out charcoal and/or letting some macros grow out a bit more might help. With a large number of fish, it would make sense to maximize the filtration capacity of your refugium, so it may help to let it get shaggy.
Happy 40th Anniversary! That's a milestone to be very proud of.
Very cool that you collected some stuff for your tank. More bio-diversity! I look forward to seeing it.