How do you quantify (count) the number of zooanthellae in a SPS?

Palani

New member
I'm getting ready to do a research project with coral bleaching with reef building corals (SPS) and I was wondering how do you count the zooanthellae in the nubbin (frag)? What techniques do you use? Are there any methods papers out there that I don't know about? Any help will be appreciated.

Shoots,

Palani
 
I think it requires those dentist microscope glasses and an abacus. Good luck!
 
<a href=showthread.php?s=&postid=9537953#post9537953 target=_blank>Originally posted</a> by Palani
I PM'ed Eric Borneman, so hopefully if he has time he will reply.
I believe Eric has some Symbiodinium slides.

He works for NOAA, does he not?
No, he doesn't work for NOAA.
 
Re: How do you quantify (count) the number of zooanthellae in a SPS?

<a href=showthread.php?s=&postid=9529614#post9529614 target=_blank>Originally posted</a> by Palani
I'm getting ready to do a research project with coral bleaching with reef building corals (SPS) and I was wondering how do you count the zooanthellae in the nubbin (frag)? What techniques do you use? Are there any methods papers out there that I don't know about? Any help will be appreciated.

Shoots,

Palani

What we commonly refer to as zooxanthellae are dinoflagellates in the genus Symbiodinium. Their population density in corals is relatively stable and varies between 1 and 5 x 10^6 zooxanthellae per square centimeter of colony surface area, which equals 20 to 60 x 10^3 zooxanthellae per small to medium coral polyp. (From Dr. Dieter Brockmann's article on photosynthesis in the February/March 2007 issue of Coral magazine. He references Sorokin (1995). Unfortunately, Dr. Yuri Sorokin's book, Coral Reef Ecology, is out of print and hard to find. I sold my copy last year.)

For anything on corals, always check with the Australian Institute of Marine Science. They do a tremendous amount of research and many of their papers are available online. They have done extensive research on coral bleaching.

Two leading U.S. experts in the field of Symbiodinium research are Andrew C. Baker and Todd LaJeunnes. Just run those two names through a Google scholar search.

:D
 
1-6 billion huh? Nice! I'll look through those papers. Since I'm on Spring Break, I'll stare at small lettering instead of T&A. Thanks for the reply. Eric still has not responded to my PM so instead I'll just read to myself.
 
<a href=showthread.php?s=&postid=9554875#post9554875 target=_blank>Originally posted</a> by Palani
Eric still has not responded to my PM so instead I'll just read to myself.
For Symbiodinium research, Eric is going to refer you to Andrew C. Baker and Todd C. LaJeunesse. I discussed this topic with him just last week.

You might find this lecture by Andrew Baker interesting. I read a paper by Baker (2003) last week but I evidently didn't save a link to it and now all I'm coming up with is the abstract and a notification that I don't have access.

Just do an advanced Google scholar search using Symbiodium as the search term and AC Baker or TC LaJeunesse as the author. You will get lots of hits.
 
Thanks Ninong. I emailed the Australian Institute of Marine Science about this so I just have to hear from them. I'll take a look at this lecture and google Baker and LaJeunesse. Thanks!
 
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