Not sure on the Hiatt. Did the folks at Seachem say after the 24 - 48 hours the ammonia will release back in? I have read on the Kordon website regarding Amquel, that it changes the structure of the ammonia molecule, therefore making it non-toxic....it's still there buy not toxic. One interesting note, is that the API test kit will give false high readings, so I had to switch to Hagen. They say the Nessler style reagents will give false readings, however the API which does not use Nessler still gives false high readings (according to warning label on Amquel). Seachem I am sure is a different formulation, so I am unsure about the ammonia readings on your test kits.
I believe what Seachem is saying is that after dosing the Prime is still available to detoxify ammonia for 24-48 hours, then is inactive. NOT that it will unlock the ammonia (become toxic again) after that timeframe. In either case, if you dose, you should be able to see a difference VERY shortly after with your test kit. If you get to zero, then you may have some active Prime still in your system that can lock up ammonia for the next 24 hours or so. If you still have ammonia showing up, then you have no more Prime availalbe, it has been used up.
Your HOB filter should become active soon. A PolyFilter somewhere in your flow path should be very beneficial, and since your tank is only 10 gallons, may last a while before turning dark brown / black.
SV