<a href=showthread.php?s=&postid=14363152#post14363152 target=_blank>Originally posted</a> by ai-reefer
They can survive without a host. Any marine biologist will tell you this.
They can survive without a host, (I'm a marine biologist

)
but, they cannot complete their life-cycle without one, as Cryptocaryon is what's called an obligate parasite. If left without a suitable host for a long period (weeks), it is generally thought that the parasite will no-longer be viable.
This paper defines the organism as an obligate parasite, and they were only able to complete the life cycle by using a "surrogate" media consisting of...cultured fish cells.
Hobbyists, farmers, lfs owners, wholesalers, etc. have used this feature of its life-cycle in their favor to rid systems of the parasite, which is probably why we find many people suggesting it as a good practice. I have seen a single study that suggest the parasite survives in the interstitial spaces within arthropod skeletons, etc, but I don't find it convincing, as it hasn't been replicated or seen anywhere else that I know of, and of course I conveniently can't find it right now
During the time that the infected system is allowed to run without suitable hosts, the fish are treated to kill the infectious stages of the parasite by using chemical therapies or prolonged hyposaline conditions. etc.
I can't remember the paper exactly, as it's been a while, but I do remember that researchers had difficulty studying this parasite because the cell-lines tend to break-down when kept in closed systems for prolonged periods. During the study, they lost the ciliate cultures after a few generations and found that they were no-longer able to infect naive fish (with no previous exposure). This might suggest that simply letting the parasite run its course for several generations without adding any "new blood" will cause it to die out. I think this is the abstract for that paper
here, but I don't have access to the university journal system anymore, so can't read the whole thing

The paper is mentioned
here with the following quote being the interesting part for us:
"Burgess and Matthews (1994) were attempting to maintain a viable population of C. irritans which could be used in later studies. To maintain the parasite populations, they needed host fish in order for the trophonts to feed and continue the life cycle. Each host fish was only used once in a process of serial transition such that none of the hosts would die or develop an immunity. While the procedure worked very well and enabled them to maintain populations for some time, the viability of the populations decreased with time and none of the 7 isolates they used survived more than 34 cycles, around 10 to 11 months. They suggest this is due to senescence and aging in cell lines is well recognised in Ciliophora."