Hi,
I have a 15g tank filled with 7" of aragonite, as rdsb, and I wonder if I replace the aragonite with siporax, will achieve the same or more denitrification by heterotrophic bacteria? Is there any research except anectodal evidence, that denitrification , do occur in siporax? How much siporax/Lt of tank and what flow through it, did you use to achieve denitrification? Thanks in advance for any kind of information.
Nitrate is decreased by bacteria, with two ways.
A) If dissolved oxygen and nitrate available in water, bacteria uses oxygen to respirate and nitrate to build more bacteria bodies (Aerobic conditions).
B)In lack of oxygen, bacteria starts to use nitrate (NO3) to get the oxygen in it, for respiration, and N2 comes out in gas form, in the end of reactions (aneorobic conditions).
Since siporax is a good bed for bacteria colonies, bacteria quickly fills its pores and establishes.
Then the problem starts. Bacteria needs food. And bacteria food is simply called carbon. There are (again) two ways to carbon-feed the hungy waiting bacteria around siporaxes:
1) You artificially introduce carbon source to siporax container (methanol works here, not sure about other carbon sources, they probably work), and pass aquarium water through siporax for bringing more nitrates to them, and during this circulation, you bring dissolved oxygen, also. In this method, relatively higher circulation is generally preferred. Plenty of oxygen, nitrate and carbon results with huge amount of bacteria production, which uses nitrate in water to build theirselves (see A above)
2) You let bacteria use the existing carbon (which will not be as high as above) within the water column, by passing the aquarium water through the siporax container. But in the second method, along with carbon and nitrates, you carry dissolved oxygen to siporax, too. In here, structure of siporax container, flow speed, oxygenation status of water reaching to siporaxes, amount of useful carbon in water column etc etc may alter the percent of the ways of nitrate processing (A or B), they probably occur together, with varying success.
As indicated by others, outer and inner parts/surfaces of both siporax mass and individual siporaxes may function differently to process nitrates.
Since siporax arrangement and water properties are different in every aquarium, success from siporax use will also vary. What I see from people's experiences is, passive (no external carbon) use of reasonable amount (like 10 liters or less) of siporax is successful if nitrate levels are already low, like around 5. To deal with high nitrates or heavy feeding habits, examples start from 50 liters of siporax and goes through 150 or more liters, requiring seperate (remote) containers.