Microfood culture: phytoplanktons, Rotifers, ciliates, Artemia, and copepods
- Thread starter FMarini
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Email Erik at Algagen: eriks@algagen.com
Do you have a good cutlure of microalgae? You'll need at least Iso; but would do better with a diatom as well. Nanno won't work.
Do you have a good cutlure of microalgae? You'll need at least Iso; but would do better with a diatom as well. Nanno won't work.
Teremei
New member
I just got myself a nice fancy stand mounted hatchery with drain on the bottom. I was so excited, I started my first culture. They are just starting to hatch now. If I read that guide right, I shouldn't even worry about feeding them for another 8 hours, because their mouth and anus aren't fully developed. Is this correct?
This is baby brine by the way. .
This is baby brine by the way. .
mwp
In Memoriam
There's probably stuff like this already in the thread, but hey, maybe not? Below are some pix and vids I shot with the QX5 for the Mandarin Thread (http://www.reefcentral.com/forums/showthread.php?s=&postid=7740361#post7740361) - in a nutshell, I sampled 1 L-Strain Rotifer Culture and 1 SS-Strain Rotifer Culture. Seived both through 53 micron and seived the flow-through in a secondary 10 micron seive. It's really worth noting the amount of TINY life prospering in the L-Strain Culture; meanwhile the SS-Strain culture had virtually NOTHING other than rotifers....not even worth showing the pics and vids of what was collected via the 10 micron from the SS-Sample...again, literally NOTHING.
One other note, I chilled the samplings in the freezer for a minute or two to SLOW these tiny creatures WAY DOWN...normally they're almost a blur under the microscope, especially at 200X.
Videos are .avi; roughly 1 to 2.5 mb a shot.
L-Strain Rotifers at 60X - numerical measurments are 1 MM
Video - http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_60X_chilled.avi
SS-Strain Rotifers at 60X - numerical measurments are 1 MM
Video - http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_60X_chilled.avi
L-Strain Rotifers at 200X - numerical measurements are 200 um (microns, micrometers etc..)
Video -
http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_200X_chilled.avi
SS-Strain Rotifers at 200X - numerical measurements are 200 um (microns, micrometers etc..)
Video - http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_200X_chilled.avi
L-Strain Culture, smaller than 53 microns but larger than 10 @ 60X:
Video - http://www.cichlidrecipe.com/nanoreef/L_Strain_less53micron_60X_chilled.avi
FWIW,
Matt
One other note, I chilled the samplings in the freezer for a minute or two to SLOW these tiny creatures WAY DOWN...normally they're almost a blur under the microscope, especially at 200X.
Videos are .avi; roughly 1 to 2.5 mb a shot.
L-Strain Rotifers at 60X - numerical measurments are 1 MM
Video - http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_60X_chilled.avi
SS-Strain Rotifers at 60X - numerical measurments are 1 MM
Video - http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_60X_chilled.avi
L-Strain Rotifers at 200X - numerical measurements are 200 um (microns, micrometers etc..)
Video -
http://www.cichlidrecipe.com/nanoreef/L_Strain_Rotifers_200X_chilled.avi
SS-Strain Rotifers at 200X - numerical measurements are 200 um (microns, micrometers etc..)
Video - http://www.cichlidrecipe.com/nanoreef/SS_Strain_Rotifers_200X_chilled.avi
L-Strain Culture, smaller than 53 microns but larger than 10 @ 60X:
Video - http://www.cichlidrecipe.com/nanoreef/L_Strain_less53micron_60X_chilled.avi
FWIW,
Matt
Frank do you know what they were culturing and feeding the Flame Angel larvae in Hawai ?
I am told they were half the size of rotifers. Personal communications with Martin Moe said his efforts were stalled out not having anything smaller then rotifers the first several days. He even spent time trolling with a plankton net trying to get enough small plankton to feed. But they starved out.
Any suggestions ?
BTW, a simple culture system I ran across at Scripps used f gal water jugs with bottoms removed, taps siliconed in the necks with airstones, and mounted in rack to make arrays, great for phytoplnakton, rotifers and artemia. You can grow a lot in this system. MUST keep rotifers from contaminating the phyto !
jake
I am told they were half the size of rotifers. Personal communications with Martin Moe said his efforts were stalled out not having anything smaller then rotifers the first several days. He even spent time trolling with a plankton net trying to get enough small plankton to feed. But they starved out.
Any suggestions ?
BTW, a simple culture system I ran across at Scripps used f gal water jugs with bottoms removed, taps siliconed in the necks with airstones, and mounted in rack to make arrays, great for phytoplnakton, rotifers and artemia. You can grow a lot in this system. MUST keep rotifers from contaminating the phyto !
jake
Luis A M
Premium Member
Hi Jake,you´re the same ol´Jake of BR list?.
Yes,the species of pods Baensch (and other Hawaiians) used were posted,published lots of times.One of his articles in FAMA gives names and pics.
I always felt Martin deserved to be the first to crack pigmies.He worked a lot with cherubs in BR days and he was using pods.And he knows how to use them,how you think he raised large angels in the 70´s?.
Yes,the species of pods Baensch (and other Hawaiians) used were posted,published lots of times.One of his articles in FAMA gives names and pics.
I always felt Martin deserved to be the first to crack pigmies.He worked a lot with cherubs in BR days and he was using pods.And he knows how to use them,how you think he raised large angels in the 70´s?.
laval food for centropyge
laval food for centropyge
Hi Luis
yup, same ol same ol, but been out of the country awhile,
just getting re-setup,
I am sure Martin would have been first but it conflicted with getting his book finished and to the printer so he couldnt put the time into them that he wanted to,
do you know which back issue of FAMA ? I am ordering some anyhow. I'll see what I can find here.
how are your projects doing ?
jake
laval food for centropyge
Hi Luis
yup, same ol same ol, but been out of the country awhile,
just getting re-setup,
I am sure Martin would have been first but it conflicted with getting his book finished and to the printer so he couldnt put the time into them that he wanted to,
do you know which back issue of FAMA ? I am ordering some anyhow. I'll see what I can find here.
how are your projects doing ?
jake
Haven't ever done it so can't really comment but looks like copepod culture wouldn't be too much of a nightmare. How would one go about separating nauplii from other stages? Would you just have to maintain a very large culture and then strain through suitably sized media to get suffient numbers of sufficiently small nauplii?
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