<a href=showthread.php?s=&postid=12660102#post12660102 target=_blank>Originally posted</a> by Lifes A Beach
I also use the s-strain rotifers. You should only add enough phyto or nanno to tint the water a light green. Once you get your cultures going, you'll be able to see the difference in the water color as the rots consume the greenwater. It becomes less green (clearer). Then you can adjust how much you'll need to add. I usually add a little twice a day rather than one big addition. As previously mentioned, you do not want the water to ever be clear - your rots will die.
For aeration on the rots, I use a plain rigid airline (no airstone) and just a small amount of bubbles. Someone else described it as "being able to count the bubbles slowly" so you don't want turbulent water! I guess it would be maybe 2 bubbles per second? Not very much (in Joyce Wilkerson's book, she explained that too much bubbling would actually injure the rots). Just enough to slowly turn the rotifers in the water.
If you have fairly good eyesight, you shouldn't need a magnifying glass to see the rots. Shine a small flashlight from below the water line and aim it slightly up toward the top and you'll be able to see the rots in the water.
Hope this helps!
--Barbara