Phytoplankton from frozen?

[doctorgori]

I realize I could try this 1st, but time is short:
Can you revive a phytoplankton culture from frozen? I've stored some cubes to feed rotifers and was wondering if you can start a culture from those...

Hopefully I'll get a fast answer (trying to save time & money & a trip to the LFS)

 

[reefstew]

I would guess no. You can keep it in the fridge & restart it.

 

[doctorgori]

...shoot then, I contaminated my green water; somehow the rotifers got in it...

 

[dela]

Are you just running one culture? I have two of each type of algae and a couple of times have had to split the spare culture to replace the one that crashed.

What are you feeding?

Along the lines of what you were asking, I was wondering if you could force a rotifer culture to go into crash mode, freeze it, and then revive the culture. As a back-up... to the back-up!

 

[Petshop.boy]

Hello,
so i understand right, its possible to restart a cultur from frozen phytoplankton?

I never tryed the frozen-plankton.

greetings matt

 

[jeff@zina.com]

No. The freezing process bursts the cells and kills them.

Jeff

Sent from my DROID RAZR using Tapatalk

 

[doctorgori]

Are you just running one culture? I have two of each type of algae and a couple of times have had to split the spare culture to replace the one that crashed.

What are you feeding?

Along the lines of what you were asking, I was wondering if you could force a rotifer culture to go into crash mode, freeze it, and then revive the culture. As a back-up... to the back-up!

I'm feeding corals both phytoplankton & rotifers....
I was trying to save a few steps but perhaps not
As for reviving rotifers...Dunno if rotifers can be froze but I do know moina can restart after a winter

 

[NirvanaFan]

Why not just run your greenwater through a rotifer sieve and filter them out? Sterilize the container (or use a new one) and the tubing.

I've contaminated a few nanno cultures, but if you filter the rotifers out, you should be able to get the culture going again.

 

[doctorgori]

Why not just run your greenwater through a rotifer sieve and filter them out? Sterilize the container (or use a new one) and the tubing.

I've contaminated a few nanno cultures, but if you filter the rotifers out, you should be able to get the culture going again.

I have a so called rotifer screen but "something" still gets through...I wanna say its another/smaller type of rotifer, but I dunno... sounds plausible and definetley worth a shot

..not to go off on a tangent, but I accidentally used old tankwater and it stall made green water, but with something else totally in it...these wwere obviously some kinda pod, but they did not multiply fast enough to crash the culture....

 

[Petshop.boy]

U ever tryed to boil the water bevore use it for your plankton ?
Maybe this works better.

 

[NirvanaFan]

using old tank water will definitely have all sorts of stuff in it. I use RO/DI water so I don't have to bother boiling it.

If your rotifers are that small, try running the greenwater through the sieve on two or three consecutive days. Do you know what micron your screen is? My 53 micron has no problem catching even my smallest rotifers.

Make sure you sterilize the container and the tubing. I like to use a good bleach bath, but running it under ridiculously hot water should work too. Just rinse everything well. Make sure to get the inside of your tubing as well.

 

[doctorgori]

I got the screen from JehmCo a while back, it was for rotifers originally I think, I'll hav to ask about the mesh siz

 

[GreshamH]

No. The freezing process bursts the cells and kills them.

Jeff

Sent from my DROID RAZR using Tapatalk

Too broad of a brush there as you can in fact freeze nanno and bring it back. Tet, Iso, Pav, etc. nope, but nanno, yes.

 

[GreshamH]

I'm feeding corals both phytoplankton & rotifers....
I was trying to save a few steps but perhaps not
As for reviving rotifers...Dunno if rotifers can be froze but I do know moina can restart after a winter

If you stress the rotifers, they will produce resting cysts. I doubt you could get resting cysts by freezing them so quickly though.

Pretty sure that is the case with Miona sp. as well.

 

[doctorgori]

Too broad of a brush there as you can in fact freeze nanno and bring it back. Tet, Iso, Pav, etc. nope, but nanno, yes.

that is indeed interesting news ...
Can you provide testimony...I'm asking not in a "I need proof and I'm a skeptic way" (I did ask, I am the OP)
..but in a "it sounds interesting way, tell me more"

 

[GreshamH]

that is indeed interesting news ...
Can you provide testimony...I'm asking not in a "I need proof and I'm a skeptic way" (I did ask, I am the OP)
..but in a "it sounds interesting way, tell me more"

I just did, we've done it numerous times. Nanno has a supet tough that way. Just out of curiosity sake we have over the years done it, and have had numerous customers do the same.

I work for Reed Mariculture (Instant Algae, Reef Nutrition, APBreed, etc) the worlds largest producer of marine phytoplankton concentrates, we invented the business in fact

 

[Luis A M]

I just did, we've done it numerous times. Nanno has a supet tough that way. Just out of curiosity sake we have over the years done it, and have had numerous customers do the same.

I work for Reed Mariculture (Instant Algae, Reef Nutrition, APBreed, etc) the worlds largest producer of marine phytoplankton concentrates, we invented the business in fact

Hmmm,I might be skeptic then:sad2:
"Official"knowledge says that it can not be done,freezing common aquaculture algae and bringing them back to life.
http://www.marinebreeder.org/forums/viewtopic.php?f=283&t=6604
Has your finding been published somewhere?.I remember I asked Randy Reed,the owner of Reed Mariculture,and he didn´t know if that could be done.
But being so simple to try,could you detail how you do it,so skeptics will try it and cease to be so?

 

[GreshamH]

Hmmm,I might be skeptic then:sad2:
"Official"knowledge says that it can not be done,freezing common aquaculture algae and bringing them back to life.
http://www.marinebreeder.org/forums/viewtopic.php?f=283&t=6604
Has your finding been published somewhere?.I remember I asked Randy Reed,the owner of Reed Mariculture,and he didn´t know if that could be done.
But being so simple to try,could you detail how you do it,so skeptics will try it and cease to be so?

Randy Reed (RMI shareholder) has never grown phytoplankton so he'd not be the one to talk to Tim Reed (founder)or Dr Eric Henry would be the ones since Tim runs the farm, and Eric is our resident Phycologist.

We did not do it as a trial so its not published anywhere and we'll not give the subject much attention other than what I wrote here. You are more then welcome to try it yourself Luis, you have the skills and facility to do it From viewing your link it appears you never tried Nanno?

 

[GreshamH]

Sorry, I was cranky when I wrote that. I have asked our Phycologist if he can run a quick trial for you. FWIW this is using our Nanno3600, not a frozen culture.

 

[Luis A M]

Honestly,I didn´t try NAN.But I will now!
Now,what you are saying is that Reed´s NAN frozen pastes are ALIVE!:eek1:
This will prove as remarkable news as well as surprising.The subject of the living (or not) condition of IA frozen pastes has been widely discussed over the years,since they were introduced to the market.That old dicussion rose sometimes to bio-philosophical heights addressing what life is.:rolleye1:
But the current belief is that frozen pastes are "not living" and that they can´t be used to start cultures.I believe this is sustained by the Company as well.
Nevertheless,IA NAN paste,3600 and now the new products,are widely used by clown breeders for the "green water" technique.Even if non living algae cells won´t process NH3,but contribute to it´s build up in larval water.Which doesn´t happen.Furthermore,I sometimes forget a pitcher of it in the warm fish room overnight,and it still smells fresh in the morning.As if it was alive.
Now,if NAN containing IA pastes are alive as Gresh says; how come the company has resigned the strong market tool of stating that these products are "LIVE"?:uhoh3:I´m sure many more people will use them,knowing they are not buying dead algal cells?