I set up a 75 gal. With about 50lb established live rock, (I added 30lb of rock but it was dry.) 80lbs of new live sand and a large bottle of bio spira. I have no detectable levels of any of the 3. The tank has been up fully set up for a week, with also a few days prior of the rocks sitting in the tank filled so I could reclaim my buckets. (Rocks were housed in buckets after the old tank seam blew.) do you think it's safe to move the fish from their temporary holding tank and place them in the new tank now?