It is important to remeber that I am moving the entire system into a new tank. As such, the sand is going to get disturbed there is just no way around it. I understand all of the reasons one might want to stir their dsb and why that may not be a good idea... but it is going to happen in the case.
WOW !! that most definetly changes things - I must have missed this, I thought this was a operating tank with potential DSB issues...
I'd start fresh (new or 'cleaned' sand) if your DSB is more than 5" deep. If it's only 3 or 4, I'd drain the tank and scoop the sand, tryin to keep it intact..... *sigh*... ya know.... never mind - 5 year old DSB,..... I'd clean it and only reserve a few containers to seed from.
Sorry - changing tanks really changes things. Cleaning it in a large tub with tap water (don't use tank water & vinegar as the buffer will react with the vinegar making salt/H2O before it even gets to the sand) and vinegar would be best, but only in that you'll save the money of buying new sand.
Just moving the sand over directly is a realistic option, rinsing it in tank water is even better...
IF you want to start from scratch (no life in sand), then continue with the vinegar cleaning.
This is going to make a mess you know
If you took a rubbermade bin/tub (like a 45gal from HD) and put your sand in it, say 6" deep, then rinse with tap water (hose). Once the sand appears clean, poor in 1/4 gal of Vinegar into the last batch of rinse water and sand (visualizing the water to be 1" to 2" above the sand level) and stir.. once it's reacted a while, give it a couple good rinses. You'll have to experiment with how much vinegar to use and how long to let it sit, but that's were I would start.
<disclaimer> You are going to have to play this by ear when you actually get started.. too much vinegar/too little water/too long, will leave you with less sand than you started with.... Do this at your own risk as I've NOT cleaned sand this way, only LR and CC</disclaimer>
Best of luck,
John.