Some things one has to know before starting dosing carbohydrates.

Belgian Anthias

New member
Carbon dosing has been successfully used in aquaculture for some decades now, biofloc technology has been subject of a lot of research and the process is well understood. Such a monoculture is harvested if growth stops and a new culture is started up, something we do not intend keeping a reef aquarium.


Carbon dosing in a reef aquarium has a lot of caveats and side effects that are not well understood or ignored. ref: http://www.baharini.eu/baharini/doku.php?id=en:makazi:het_water:filtratie:vodka


r strategists bacteria may grow very fast once out of lag phase and come in log phase, as growth is logarithmic. They will grow at least 5x faster as competitors. Determent for the growth rate is the nitrogen source used. Fast growth needs ammonia-nitrogen. Bacteria prefer ammonia to grow, not nitrate-nitrogen. They will outcompete other organisms for nutrients. A lot of protein is produced but logarithmic growth is always followed by logarithmic decay. ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:bio-chemie:bacteriën

Normally organic carbon for growth is retrieved from organic matter which is remineralized, producing new building materials for new growth and the carbon is released as CO2 to close the carbon cycle and to be used by autotrophs to produce organic matter with the by remineralization provided building materials. Nature limits organic carbon availability for a reason!

By adding free supplemental organic carbon the bacteria do not need to use decaying organic matter to retrieve their carbon demands and for fast growth they use up what is available in the water column. Slower growing species as autotrophs will not be able to compete. The previously installed autotrophic carrying capacity may be lost and replaced by growth which means a certain growth rate must be maintained to support the bio-load.

+- 40 x more bio-mass must be produced to reduce the same amount of ammonium. Carbon dosing also does deplete alkalinity as primary ammonium is used as a nitrogen source! ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:het_water:ammonium_reductie

What if for some reason growth stops suddenly due to insufficient supply of one or more essential nutrients or dosing is stopped abruptly?

A skimmer removes nutrients constantly but very selective, only +- 30% of organic waste, DOC and TOC, sometimes called "the nasty things" will be removed, the rest stays and must be remineralized. A skimmer is not very effective in removing DOC and TOC! ref: http://www.baharini.eu/baharini/doku.php?id=en:makazi:het_water:filtratie:eiwitafschuimer If skimmate increases due to dosing one may get an idea of what is leftover in the tank.

Corrals make use of their own private carbon cycle in the coral holobiont, this way managing their nutrient supply. ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:theorie:koraal_holoboint
I would not like to be that coral which has to compete with those r-strategists fed with an overdose of free organic carbon!

It has been shown a high C:N ratio will mess up the entire system and the presence of unused carbohydrates may kill corals. Phosphorus starvation has been shown to be the main cause of coral bleaching, dosing organic carbon, based only on the nitrate level may kill corals.

Fast growing bacteria use ammonium -nitrogen, not nitrate, and need phosphorus and other essentials. Most users of carbon dosing use it based on the nitrate level which logically can not be used as a reliable reference for dosing, as may be the daily nitrogen overproduction and or phosphorus availability. The N/P ratio for bacterial growth needed in weight is 10/1 if nitrate and phosphate are used as a reference.


As was shown carbohydrates dosing may kill corals and will for sure mess up the coral holobiont!


Vinegar is often used to improve kalkwasser addition. In that case, carbohydrates are added based on the evaporation rate of the tank, often without taking into account anything else.

In the past 2 decades a lot of elements are added to the side effects of carbon dosing. As long they are not recognized to be safe, unsafe elements eliminated or proven to be not correct one should not add carbohydrate-based supplements to a reef aquarium.

In 2008, Ken S. Feldman and Kelly M. Maers warned about increased organic carbon availability. This is more a decade ago! In an article published in the Advanced Aquarist, they wrote: "œcircumstantial evidence encouraged Rohwer et al. to speculate that the introduction of carbohydrates (= a fuel source) will generate an imbalance in the bacterial component of the holobiont and that this departure from equilibrium leads to coral mortality. The mechanism by which this induced bacterial proliferation causes coral death remains to be elucidated, and this pivotal issue must be addressed before this intriguing hypothesis can gain further traction. Nevertheless, the empirical evidence can be construed to support a robust relationship between elevated DOC levels, correspondingly increased bacterial populations, and coral mortality." They also wrote:"œ Reef bulletin boards all too frequently contain threads that begin "œHelp! My tank is crashing; my corals are dying, but all of my measurable water parameters are within expected ranges. What's wrong?" Could it be possible that elevated levels of DOC, for whatever the reason, are contributing to, or even causing, the coral loss? (FeldmanEnMaers2008>Feature Article: Total Organic Carbon (TOC) and the Reef Aquarium: An Initial Survey, Part I". Page. . https://www.advancedaquarist.com/2008/8/aafeature3.) ref: http://www.baharini.eu/baharini/doku.php?id=en:makazi:het_water:filtratie:vodka

The latest decade a lot of research has been done and confirmed the worry's published by Ken S. Feldman and Kelly M. Maers in 2008. ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:het_water:filtratie:vodka

Who would add something to a thank without knowing it improves or harms the life of the residents ?

In most cases, carbohydrates are used to lower the nitrate level. As far as I know the nitrate level never did harm or influence corals the way to much carbohydrates are able to do. The cure may be worse as the disease. What about controlling algae growth by using carbohydrates without taking into account the essential parameters present?

Assuming enough carbohydrates are dosed in a reef aquarium for directly lowering the nitrate level by heterotrophic bacterial metabolism, I would not like to be one of the corals trying to manage their coral-holobiont. In that case most ammonium will be used up by heterotrophic bacterial growth, this way removing the nitrogen source for slow-growing nitrifying bacteria, removing the previously installed autotrophic carrying capacity. This is very important, if for some reason dosing organic carbon is stopped suddenly or growth is limited by available nutrients, the carrying capacity may not be supported for weeks and ammonia may build up. Therefore it is advised to build off dosing over a period of several weeks.
 
Good info, thanks for sharing.

I take it you prefer using old school anaerobic denitrators where the bacteria is contained in a reactor?

Before all the carbon dosing I remember people using mobile buckets of sand as well for nitrate reduction

My biggest issues with higher nitrates was browning of acropora corals and also had problems with pest algae at high levels of nitrates that would create a ratio(P04 to N03) more conducive for the algae to proliferate
 
Propper nitrogen management

Propper nitrogen management

Propper nitrogen management is essential.
Passive or active?

I prefere to manage nutrients using photo autotrophs in a refuge. This way the nitrogen and or phosphorus balance is easily managed by using for nitrogen and or phosphorus modified F2media. As it is slow, it will never deplete essential nutrient availability or completely mess up the existing balance. it will not harm corals. Using a refuge active nutrient management is possible. By using modified F2 media all essential nutrients can be managed. ref: http://www.baharini.eu/baharini/doku.php?id=en:makazi:chemie:guillardf2
As most modern reef tanks have a very low carrying capacity and only are able to support a very low bio-load compared to "old school" high nutrient systems a refuge with photo-autotrophs will do fine to restore the nutrient balance. To increase the carying capacity a simple small biofilter can be added.
For high nutrient mixed reef systems with an increasing bio-load other and bigger biofilters may be used making safe active nutrient management an easy task.

Nitrate does decrease the growth rate of organisms using it as a nitrogen source by a factor +- x8. It does not proliferate algae growth!! it does not kill corals!! ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:chemie:calcificatie#calcificatie_bij_koralen
Ammonia not nitrified may be used for algae growth increasing the growth rate.
Nitrate is a safely stored nitrogen reserve which is only used when ammonia is used up but is very important for survival at that moment and is essential to complete the nitrogen cycle.
In a nitrifying biofilm +- 16% of the processed ammonia may be removed as nitrogen gas as in every nitrifying biofilm denitrification takes place. No nitrification = no denitrification!!!
Most reef aquaria use a skimmer that selectively and constantly removes building materials but not inorganic nutrients by which nitrate may build up and an unbalance between nutrient availability is created. Adding organic carbon does not solve this unbalance as metabolism needs all essential nutrients in its natural balance. Minor essential nutrients may be depleted.
Nitrate availability is easily managed without adding carbohydrates!!
Doing so can create a situation that is much worse for the health of corals than the presence of nitrate ever will.
High availability of nitrate-nitrogen has been shown to inhibit the growth of corals as is the case for all organisms when used as a source of nitrogen. ref: ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:chemie:calcificatie#calcificatie_bij_koralen
High availability of nitrogen during periods of increased growth ( temp?) can be responsible for coral bleaching when this growth can suddenly no longer be supported by the availability of other essential building materials. It is shown that a deficiency in phosphorus supply is a major cause of coral bleaching. This can also be caused by too high availability of free organic carbon, introducing too high no longer controllable fast heterotrophic growth in the coral holobiont.ref: http://www.baharini.eu/baharini/doku.php?id=nl:makazi:theorie:koraal_holoboint

Dosing carbohydrates only based on the nitrate level may harm even kill corals.
 
denitrators?

denitrators?

Good info, thanks for sharing.

I take it you prefer using old school anaerobic denitrators where the bacteria is contained in a reactor?

I do not prefer so called,"old school" denitrators at all. But what is "old school" about using denitrators? They are used all over the world for water treatment. Everything about the use of denitrators is known. The use of anoxic kept denitrators can not be advised to be used in a life support system.

For managing a high nutrient mixed reef system I prefer to use bio-filters.
The use of bio-filters makes easy active nitrogen management possible. For managing the nitrogen content one may make use of BADES. http://www.baharini.eu/baharini/doku.php?id=en:badess:start

BADES can be used without a reactor in normal aquarium conditions. No reactor needed. BADES-columns are very effective bio-filters, a nitrifying bio-filter that does not increase the nitrate level. A very high bio-load can be managed. When used in a refuge the nitrogen removal rate can easily be managed. ref: http://www.baharini.eu/baharini/doku.php?id=en:badess:bades:bades-kolommen
 
Pretty interesting stuff.

It would be helpful if you could sum up what your points are. As far as I can tell, you're saying carbon dosing is difficult to get just right, and otherwise harmful to reef tanks. You recommend the BADES method because it is more easily managed, to get exactly the results you want, incorporating both nitrification and denitrification.

If you could explain why one is easier to manage properly than the other and how that is achieved, in a practical, real-world aquarium-focused manner, that would help as well.

I enjoy reading this kind of information that challenges the status quo. It helps the hobby evolve.
 
I prefere to manage nutrients using photo autotrophs in a refuge. This way the nitrogen and or phosphorus balance is easily managed by using for nitrogen and or phosphorus modified F2media.

What "photo autotrophs in a refuge" do you use?

F2 media as I understand is filtered seawater mixed with sodium nitrate, - phosphate, trace minerals & vitamins. Is this what you are referring too? & do you does it?

For managing a high nutrient mixed reef system I prefer to use bio-filters.
The use of bio-filters makes easy active nitrogen management possible. For managing the nitrogen content one may make use of BADES

"BADESS: Bioilogical Autotrophic Denitrification using Elemental Sulfur System, a Sulphur Based Nitrogen Management System (SBNMS)"

How do you use this? Is this connected to your implementation of F2M ?

Some photos of your applications would be helpful Makazi.
 
I do not prefer so called,"old school" denitrators at all. But what is "old school" about using denitrators? They are used all over the world for water treatment. Everything about the use of denitrators is known. The use of anoxic kept denitrators can not be advised to be used in a life support system.

For managing a high nutrient mixed reef system I prefer to use bio-filters.
The use of bio-filters makes easy active nitrogen management possible. For managing the nitrogen content one may make use of BADES. http://www.baharini.eu/baharini/doku.php?id=en:badess:start

BADES can be used without a reactor in normal aquarium conditions. No reactor needed. BADES-columns are very effective bio-filters, a nitrifying bio-filter that does not increase the nitrate level. A very high bio-load can be managed. When used in a refuge the nitrogen removal rate can easily be managed. ref: http://www.baharini.eu/baharini/doku.php?id=en:badess:bades:bades-kolommen


Apparently the term "old school" doesn't translate well. It means a proven method that has been around for years as you have noted.

I may have simplified your BADES concept but it seems like a common denitrator fueled by sulfur additions.
Please explain more on why or how it's different.
 
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Pretty interesting stuff.

It would be helpful if you could sum up what your points are. As far as I can tell, you're saying carbon dosing is difficult to get just right, and otherwise harmful to reef tanks. You recommend the BADES method because it is more easily managed, to get exactly the results you want, incorporating both nitrification and denitrification.

If you could explain why one is easier to manage properly than the other and how that is achieved, in a practical, real-world aquarium-focused manner, that would help as well.

I enjoy reading this kind of information that challenges the status quo. It helps the hobby evolve.

I just provide information about known problems and concerns about dosing carbohydrates. There are a lot of side effects of which the impact is not known, enough doubts for not using it.
It has been shown that dosing carbohydrates and increasing the C/N ratio may harm corals. This means it is not safe to dose carbohydrates only based on the nitrate level, it may lead to essential nutrient starvation in the coral holobiont.
It has been shown the presence of carbohydrates may kill corals. This may happen when for some reason growth is insufficient to use up the daily doses within a certain period of time.

If nitrate is removed directly by heterotrophic bacterial assimilation due to dosing one may speak about an overdose messing up the balance in the entire tank.



BADES is an option if only nitrate must be removed to restore the nutrient balance, it is safe to use as all caveats and side effects are known and can be avoided or corrected. By using BADES the daily nitrate removal rate can be managed as desired, active nutrient management is possible.
BADES can also be used to increase the carrying capacity without influencing the nitrate content much.
For LNS I recommend using a refuge for photo-autotrophic nutrient assimilation.
Basic management of a life support system starts with: one does not add products of which one does not fully understand of what the outcome may be, or products of wich one does not know what they contain exactly.

Modern reef tanks are dependable of passive nutrient management, wait, see and hope.

Carbon dosing is based on the assumption the added organic carbon will be used for growth and protein production and the produced protein will be removed by a skimmer. As not one of both processes is controllable we speak of passive nutrient management. And there are a lot of other processes which are activated by increased organic carbon availability of which we do not have control.

Active nutrient management is having control over the processes in mind.
 
About BADES?

About BADES?

Apparently the term "old school" doesn't translate well. It means a proven method that has been around for years as you have noted.

I may have simplified your BADES concept but it seems like a common denitrator fueled by sulfur additions.
Please explain more on why or how it's different.

A common denitrator is kept anoxic. DO below 0.5ppm. The concept was taken over from carbon based denitrators. BADES does not need anoxic conditions. A common denitrator does not increase the carrying capacity!

BADES is not my concept. BADES is used for nitrate removal in marine aquaria for more than 25 years. ref:http://www.baharini.eu/baharini/doku.php?id=en:badess:bades:maao

Reactors used in BADES Systems are not kept anoxic! Also in MAAO the reactors were not kept anoxic. Hignette publishes in 1996:" it works fine at a DO of 3ppm". ref:http://www.baharini.eu/baharini/doku.php?id=en:badess:bades:maao The nitrate removal rate is easily managed by adjusting the flow rate, as desired.

For the applications of BADES no reactor is needed. ref: http://www.baharini.eu/baharini/doku.php?id=en:badess:bades:bades-kolommen

BADES can be used in a reactor, in a refuge, in a nitrifying biofilter, in a substrate, improving Jabert etc...
BADES biofilm reactors and BADES-columns are biofilters for ammonium removal by simultaneous nitrification and denitrification and may support a very high bio-load without increasing the nitrate level. ref: http://www.baharini.eu/baharini/doku.php?id=en:badess:start
http://www.baharini.eu/baharini/doku.php?id=en:badess:bades_bio-film_reactor&rev=1583498738
BADES is not my preferred nutrient management method for managing LNS.


This tread is not about using BADES.

The thread is about information and concerns about adding carbohydrates to a reef aquarium.
 
GuillardsF2

GuillardsF2

What "photo autotrophs in a refuge" do you use?

F2 media as I understand is filtered seawater mixed with sodium nitrate, - phosphate, trace minerals & vitamins. Is this what you are referring too? & do you does it?



How do you use this? Is this connected to your implementation of F2M ?

Some photos of your applications would be helpful Makazi.

GuillardsF2 media: it is a standard balanced food supplement for the cultivation of photo-autotrophs and algae. There are many others adjusted for a specific target species. http://www.baharini.eu/baharini/doku.php?id=en:makazi:chemie:guillardf2.
Modified means that one or more essential nutrients are missing which means they must be retrieved from the water, this way lowering the target nutrient availability in the water column without depleting the others.
 
Thanks, Belgian Anthias. You pretty much answered my questions. Would you mind going into more detail about your own BADES setup? This is all new to me, so I'm curious. It sounds like there are several ways to go about it.
 
just noting that most of your links are "permission denied".

GuillardsF2 media: it is a standard balanced food supplement for the cultivation of photo-autotrophs and algae.
algae are photoautotrophs, as are photosynthetic bacteria. That's why I asked you what type of photoautotrophs you have in your fuge? I take it you are simply referring to macroalgae(s)?

F2 Media is algae food & I expect that's what Triton Core 7 additive is based on.

So I'm a little confused. You said in one post that you prefer to manage nutrients using photo autotrophs in a refuge, & you dose elements specifically to enhance growth. On another post you said that you prefer to use bio-filters - the BADES reactor utilising autotrophic bacteria - "This method makes it possible to completely close the nitrogen cycle by effectively eliminating daily nitrate nitrogen overproduction". Your aim = low nitrate.

Macroalgae prefer to assimilate nitrogen in the form NH3/4. This also closes the nitrification cycle, although perdurable nitrogen oxidising bacteria will still get a share of NH3.

The Feldman etc info in your OP is pretty well known by most hobbyists, as is the theory on N;P ratios - phosphate starvation, & to some degree the dangers of carbon dosing. I'd like to hear a more precise explaination of your set-up, in plain words. Macroalgae refugium >< BADESS biofiltartion.... both ???
 
just noting that most of your links are "permission denied".


algae are photoautotrophs, as are photosynthetic bacteria. That's why I asked you what type of photoautotrophs you have in your fuge? I take it you are simply referring to macroalgae(s)?

F2 Media is algae food & I expect that's what Triton Core 7 additive is based on.

So I'm a little confused. You said in one post that you prefer to manage nutrients using photo autotrophs in a refuge, & you dose elements specifically to enhance growth. On another post you said that you prefer to use bio-filters - the BADES reactor utilising autotrophic bacteria - "This method makes it possible to completely close the nitrogen cycle by effectively eliminating daily nitrate nitrogen overproduction". Your aim = low nitrate.

Macroalgae prefer to assimilate nitrogen in the form NH3/4. This also closes the nitrification cycle, although perdurable nitrogen oxidising bacteria will still get a share of NH3.

The Feldman etc info in your OP is pretty well known by most hobbyists, as is the theory on N;P ratios - phosphate starvation, & to some degree the dangers of carbon dosing. I'd like to hear a more precise explaination of your set-up, in plain words. Macroalgae refugium >< BADESS biofiltartion.... both ???

The aim is to balance the nitrogen content, to restore the unbalance created by the use of a skimmer. This limits the risk of nutrient starvation during periods of increased growth. This has nothing to do with the so-called "theory of N:p ratio's" but with the sensing mechanisms of organisms and their preference for nutrients and possible growth rates, the availability of nutrients where the action is.

My preference depends of the kind of system to manage, the required carrying capacity to support the intended bio-load, now and in the future.

guillard's f2 is only one of the many standard food formula for the cultivation of photo-autotrops for aquaculture. It is available in many modified forms. it Is not a brand. Many commercial brands may be based on this formula.

This tread is about carbon dosing and the possible side effects!
 
Access denied!

Access denied!

just noting that most of your links are "permission denied".

If the access denied page is shown this means you have to register to enter the Makazi Baharini wiki and visite the page containing protected content. After free registration, the information is available read-only. After registration one receives a password to enter the wiki.

On the access denied page there should be the possibility to register or and enter your password.
 
Sulphur

Sulphur

Thanks, Belgian Anthias. You pretty much answered my questions. Would you mind going into more detail about your own BADES setup? This is all new to me, so I'm curious. It sounds like there are several ways to go about it.

Every system is different. All info needed about BADES and a BADESSystem, an SBNMS, is available in the Makazi Baharini wiki.
 
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