I started with 4" in my display and put close to 6" in my sump when I installed it. Seems to be working fine for me but in hindsight I would go with just enough in the display to get a good look and a remote sand bed in the sump for the de-nitrification. I like the way tanks look when they have some sand on the bottom, but why waste space for a DSB in the display when it works just as well remotely?