I have the same type of results, fairly close, as Sarah's there.
I really doubt your test kit have even remotely the accuracy and precision to measure NO3 that low. Most at best are like the Lamottes at 1 ppm maybe 2ppm etc. There are ULR test kits that measure well, but these are generally colorimeters/spects and certainly need a calibration curve and ref standards done.
Same deal with low PO4.
All the test kit methods unless specifically addressed, test total PO4 or total NO3.
Not Inorganic forms.
eg SRP PO4 is the standard method for inorganic fractions of bioavailable forms.
We might measure 0.1ppm PO4 but it's not bioavailable to the macros, pest algae might be able to cleave the bond however and use it, or the low trace ppb ranges that leach out of coral, rock, sediment, macro algae are more than enough to support growth and non limiting conditions for many species.
I really have issues when we get this low and start thinking we have such precision at such extremely low concentrations, that's very tough to measure and show in a research, let alone in a hobby.
There is not even a private lab that the SFWMD could send samples to the would guarantee 10ppb of PO4 +/- 3ppb for the indepenedent analysis in their monitoring program.
Chemostat experimenet suggest the sub 10ppb range is limiting to small noxious algae. They are a PITA to keep going also(Chemostats).
This is why looking at the upper end of the curve is so appealing and useful to hobbyists.
Far easier and from a how/management perspective, much easier and addresses good growth for far more species.
I've grown 48 species of macro algae and marine plants well.
The 2ppm of NO3 might have been removed by bacteria in a DBS.
But I think the macros got it.
The macro algae might be limited by Fe/PO4 etc, so adding more NO3 really did not do much, however, another species might have been limited by something, it might not be NO3 either.
The NO3 was low, you added some, so generally speaking, if you add one thing and not the others, the PO4 might have dropped to zero, of the FE.
Plants, macro algae all self regulate their metabolisms, so if you add say PO4, that will increase NO3 uptake by the plant. Likewise if the plant is limited by NO3, adding more PO4 will not do anything either.
Secondary effects are very common in plant phyisology and in reefs/FW planted tanks/ Agriculture etc.
They do not imply cause however.
You have to have control over the all the parameter to test the one of interest and they need to be non limiting over the duration of the test.
Then you vary the compound of interest over a wide range and and see the results.
If you had a limited NO3 system and added PO4, would you expect to see a high O2 production rate from the macro algae?
I wouldn't.
Regards,
Tom Barr