UCIrvine LA,ALA,Oleic acid,EPA,GLA,DHA,Lipase EXPERIMENT

[ikn0xl]

If there are people such that your major or intended profession is in the chemical field please do chime in, just kiddin EVERYONE is welcome to comment.

LA Linoleic Acid"Omega 6"- 422 mg
ALA Alpha Linolenic Acide - Omega 3 - 368mg
Oleic Acid - Omega 9 - 432 mg
EPA 180mg- Omega 3- 180mg
GLA- Omega 6- 180mg
DHA -Omega 3- 120mg
Lipase- 1200 units

Through chemical analysis and purified lab grade use of the above and mentioned Fatty Acid groups I have been supplementing approximately the amounts provided and
{+/- 22mg...41 units <due to not having a micropipetter around>} I have had very good results in coral polyp extension along with stable water parameters

Nitrate increased 10ppm over the course of 2 weeks
- can also be caused by increased feedings
Stable 8.75 ALK readings
475 Calcium
0 Nit,Amm,Phosphate - through the use of carbon and phosban

THe skimmer goes crazy once the mixture is added and if not fully dissolved in salt water (e.g. putting the solution in a water bottle and shaking vigerousely for 5+ minutes) the solution will only linger at the waters surface.

NO ALGAE growth that was not obsereved before starting , so far its been 3.75 weeks

I will post logs and accurate measurements within 1 week.

Teh reason for this is to increase levels of fatty acids in the tank invert population along with algae or any other organism lower in the food chain thus providing additional supplementation to other organisms higher in the food chain.

I had to do an experiment for school and this seems somthing I was interested in.

If youve had any experience with this please let me know I would like to as a few questions, ANy comments please advise.

Regards
Sev
storossi@uci.edu

 

[dolt]

sev - is that human lipase you are using? if so do you mix all this before adding to the tank for a certain time period? what prompted you to even try adding fatty acids? and why these particular ones? is there a hypothesis or something in particular you are trying to prove or measure? sorry so many questions but I find this very interesting and am interested to see what kind of results you get

 

[Randy Holmes-Farley]

What is the purpose of the lipase?

Many people dose selcon, which is a fatty acid supplement.

Are you planning to test the hypothesis that you are increasing the fatty acid content of inverts and algae? I wouldn't expect that most algae will take up fatty acids, except perhaps cyanobacteria.
Do you have information suggesting otherwise?

 

[ikn0xl]

I believe the oraganic intake of algae will not be extracting levels of fatty acids from the water column to the degree hat I can detect, Also I cannot supply a negative and positive control due to the fact that I dont have 3 tanks.

Although you strike a very relevant question , how to determine invert intake of fatty acid consumption, if any.

Also the largest factor concerning my observations is that I will be supplying other sources of nutrition also leading to the addition of supplements that may contain fatty acids and aid in the false premise that Im contribution to the tank through my cocktail.

Currently I have a multimineral and multivitamin, fatty acid "type mentioned above" ,and ro/di water cocktail that I have prepared and have soaked freeze dried shrimp, formula 1 and 2 pellets in and will be refrigerated, I will be dosing based on observation only.

You mentioned Selcon yes very popular especially among those that are having a problem with ich.

The way I will judge my decision will be though but I will attempt to conclude my efforts through the following,

1) strict observation
2) water quality
3) water column parameter stability
4) algae growth
5) macro algae growth rate-(in fuge)more nutrients the faster it grows
6) fish health
7) oxygen content in water column
8) organic content comparison to NSW
9) Coral health and rate of growth
and lastly
10) Skimmate content and consistency.

I think Ive narrowed it down to 10 determinants, please advise If you think Im waisting my time =) Id rather take your experienced word for it rather than attempt to CRASH my tank.

Thank you for your comments they are throughly read and taken into consideration.

Regards
Sev

 

[ikn0xl]

Through my understanding based on the following exerpt:

"In living organisms lipids serve as the basis of cell membranes and as a form of fuel storage. Often lipids are found conjugated with proteins or carbohydrates, and the resulting substances are known as lipoproteins and lipopolysaccharides. The fat-soluble vitamins can be classified as lipids. Liposomes are spherical vesicles formed by mixing lipids with water or water solutions."

with this in mind Lipase is added due to the following:

"LIPASE [lipase] , any enzyme capable of degrading lipid molecules. The bulk of dietary lipids are a class called triacylglycerols and are attacked by lipases to yield simple fatty acids and glycerol, molecules which can permeate the membranes of the stomach and small intestine for use by the body".

Basically to aid in the absorbtion and break down of the fatty acid,whether phospholipids are affected by the addition of lipase is my main concern because the phosphate backbone in our systems is crucial and it goes back to your main question of whether or not hte algae will consume or extract the omegas from the water column.

Do you think the use of a skimmer and filter sock will greatly ihibit any type of consumption or lysis of the fatty acids by various organelle.

 

[Randy Holmes-Farley]

Lipase breaks triglycerides, diglycerides and monoglycerides down into fatty acids and glycerol, it does nothing to fatty acids themselves. Most forms of lipase will not break down phospholipids.

FWIW, I know quite a lot about lipase as I am coinventor of a pharmaceutical in human trials that is a lipase inhibitor that prevents dietary fat from being converted into fatty acids:

http://www.peptimmune.com/gt389.asp

Humans only take up fatty acids, not triglycerides (fat). So it leads to a weight loss effect.

 

[ikn0xl]

Very good to hear, your background is not being overlooked with the above comments about Lipase, also I would like to find more information on C0-14, I am intersted in the mechanism in which it can upregulate or downregulate (i did not remember reading that) T cell signaling.

Do you have any personal experiences with that topic in particular?

If so please elaborate.

Thanks for your professionalism.

Regards
Sevan Torossian

 

[Randy Holmes-Farley]

C0-14 is what?