will it cause a cycle to remove old sand

Moonstream

New member
I am panning to remove at east 2" from the top of my sandbed, so that I only have about 1" left in the tank. I am planning to wash the old sand very well, then put it in my fuge; I was wondering if this would cause a cycle?
 
Keep in mind that the bacterial flora you grew when you cycled your tank live in your LR and sand. Sand has a super high surface area and hence the greater portion of your bacterial flora. Also, most all the nitrate converting bacteria you have are in your sand (since they are mostly anerobes). Essentially you are throwing aware a large part of your bacterial filter.
 
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