I do not remember how much I dosed. I thought you used freshwater though. Oh I remember a diluted mix of salt water. Right? It was cool doing it. I wanted to grow brin, phyto, and rotifers. One day I will start that back up. Just do what he said about replaceing your rigidtubing, airline tubing, and bottles. That is what got me. I was getting it to grow so dark and then the brine started growing in there. CRASHED Good luck keep us up to date.