http://www.advancedaquarist.com/2011/3/aafeature
CONTENTS
1. Introduction
1.1 The goal of our study - testing the validity of the Carbon Dosing hypothesis
1.2 Bacteria: A general introduction
Bacterial Physiology
Bacterial Surface Charge and Protein Skimming
1.3 Bacterial life processes
Bacterial Metabolism
Bacterial Growth
Bacterial Nutrients
Manipulating Bacterial Growth
The Coral Holobiont
"Probiotic" Application of Bacteria
1.4 Counting bacteria in the water column
2. Experimental Approach
2.1 General experimental
2.2 Control experiments and bacterial contamination
2.3 Data workup
3. Results and Discussion
3.1 Baseline bacteria counts
3.2 Carbon dosing (planned and inadvertent) - How does it affect water column bacteria levels?
3.3 Bacteria removal via mechanical filtration - how effective?
Five of the six salt mixes (Red Sea excepted) displayed little bacterial contamination beyond the pure water control, and the bacteria populations fluctuated but did not consistently rise over time as might be expected if sufficient nutrients were available (cf. Fig. 13). Thus, there is no reason to suspect that any of these five salt mixes themselves contribute to the high levels of bacteria in the make-up saltwater of Fig. 13. Red Sea salt, on the other hand, does appear to bring with it a not insignificant bacterial load. Once again, there was little increase over 5 days, indicating that sufficient nutrients for growth were not available under these "sterile" conditions. What is so special about Red Sea salt? This salt, uniquely among the six mixes tested, is made, at least partially, by drying authentic seawater. Thus, it appears to retain some viable bacteria from the drying process. The other five salt mixes are prepared from mixing strictly chemical sources of the components. Overall, it appears justified to conclude that the significant populations of bacteria in mixed saltwater are a result of container contamination and not salt mix introduction per se.
Scotty,
Please paste this in your brain.
Patrick
CONTENTS
1. Introduction
1.1 The goal of our study - testing the validity of the Carbon Dosing hypothesis
1.2 Bacteria: A general introduction
Bacterial Physiology
Bacterial Surface Charge and Protein Skimming
1.3 Bacterial life processes
Bacterial Metabolism
Bacterial Growth
Bacterial Nutrients
Manipulating Bacterial Growth
The Coral Holobiont
"Probiotic" Application of Bacteria
1.4 Counting bacteria in the water column
2. Experimental Approach
2.1 General experimental
2.2 Control experiments and bacterial contamination
2.3 Data workup
3. Results and Discussion
3.1 Baseline bacteria counts
3.2 Carbon dosing (planned and inadvertent) - How does it affect water column bacteria levels?
3.3 Bacteria removal via mechanical filtration - how effective?
Five of the six salt mixes (Red Sea excepted) displayed little bacterial contamination beyond the pure water control, and the bacteria populations fluctuated but did not consistently rise over time as might be expected if sufficient nutrients were available (cf. Fig. 13). Thus, there is no reason to suspect that any of these five salt mixes themselves contribute to the high levels of bacteria in the make-up saltwater of Fig. 13. Red Sea salt, on the other hand, does appear to bring with it a not insignificant bacterial load. Once again, there was little increase over 5 days, indicating that sufficient nutrients for growth were not available under these "sterile" conditions. What is so special about Red Sea salt? This salt, uniquely among the six mixes tested, is made, at least partially, by drying authentic seawater. Thus, it appears to retain some viable bacteria from the drying process. The other five salt mixes are prepared from mixing strictly chemical sources of the components. Overall, it appears justified to conclude that the significant populations of bacteria in mixed saltwater are a result of container contamination and not salt mix introduction per se.
Scotty,
Please paste this in your brain.
Patrick
