huig
New member
Hi Amy,
I have been thinking about cryopresevatoin of trochophores as well, I thought I was alone. Part of my work is cryopreservation of mamalian cells for longterm storage and reviving them as needed. Did you use a cryoprotectans (DMSO or glycerol)? and at what rate did the cooling take place? It could be that cells ruptured due to the formation of large kristals which occur when cooling occurs to fast.
greetings
I have been thinking about cryopresevatoin of trochophores as well, I thought I was alone. Part of my work is cryopreservation of mamalian cells for longterm storage and reviving them as needed. Did you use a cryoprotectans (DMSO or glycerol)? and at what rate did the cooling take place? It could be that cells ruptured due to the formation of large kristals which occur when cooling occurs to fast.
greetings