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what is a good way to get DNA from a live fish without cutting the fin? im guessing swabbing the mucus will yield some cells. thanks
DNA extraction methods for live fish
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my goal is to sex a porcupine puffer. i have the mitochondrial sequence which im using as a control, i still need to find a gene from a sex chromosome
Spracklcat
Disaster Expert
That would be my bet too, though a swab, especially near the mouth or vent might yield enough cells depending on your test sensitivity. You don't have to worry about bacterial contamination since bacteria don't have mitochondria. As long as your sequences are mitochondira-specific you should be fine. If it were me I'd try the swab before I tried anything invasive.
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im using PCR so only a cretin gene will will be multiplied. does anyway know of sex chromosome specific genes that are seen in all fish?
Problem is that not all fish determine sex via genetics. Some determine sex based on temperatures during certain phases of development, some based on age, some based on population densities....you get the idea 
You might want to do a literature search on Diodontidae and see if anyone has done any genetics work on the family that might be useful.
You might want to do a literature search on Diodontidae and see if anyone has done any genetics work on the family that might be useful.
FunkieReefJunkie
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One bad CSI Las Vegas Episode.
Grissom: I know who killed the fish maintenance man.
Catherine: Who?
Grissom: It was the puffer.
Nick: C'mon.
Grissom: No. I noticed the little pucker marks all over the vics body, so I swabbed the puffer's mouth at exactly 78 degree fahrenheight for DNA. I knew then the male puffer was the killer. The slime on the petechiae matched the fishes mitochondria. He puckered him to death.:lol:
Grissom: I know who killed the fish maintenance man.
Catherine: Who?
Grissom: It was the puffer.
Nick: C'mon.
Grissom: No. I noticed the little pucker marks all over the vics body, so I swabbed the puffer's mouth at exactly 78 degree fahrenheight for DNA. I knew then the male puffer was the killer. The slime on the petechiae matched the fishes mitochondria. He puckered him to death.:lol:
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I looked more and didnt find much more then i had already. this is for a science research program at school. my new goal is to find the gene for TTX resistnace (the puffers toxin) and multiply it
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malverne high school, i see 2 of you are from li too
J. Montgomery
Mouth Pipetting
Thats a very ambitious project. Unless you're already very good at cloning and know the DNA sequence of your gene of interest, then you're in over your head. It ain't gonna happen. I'd suggest finding a research project that could realistically be completed.
I'm a PhD candidate in Cell Biology, and that would be an intimidating task for any PhD level research scientist, much less a high school student.
For starters, you don't really clone stuff from chromosomes. You'd need to isolate the messenger RNAs (b/c you can't have introns in your cloned gene), and then use those to generate a cDNA library. You'd need to know your sequence of interest in order to synthesize PCR primers, etc . . . it's exponentially more complicated than you'd think.
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im not trying to find the sequence i have it already i just want to use PRC. iv done this with my own genes and ran a gel. i just wanted to get dna from my puffer and not hurt him
