You are correct! I didn't specify I was looking for something a little more clown-friendly.
However.... I also didn't specify that you got to choose the beer!
DOH! I'm getting Milwaukie's Best, aren't I...? Please, PLESAE no Natty Light!
Question - I'm assuming the first photo is of larval/planular stage(?) Did you try to get them to settle on substrate? Or did you just use an empty glass container? I have heard of selective settlement and issues with metamorphosis if you don't have the right substrate.
Yes, and they were in that planula phase MUCH longer than I expected - almost a month before I saw any settlement. I honestly thought they had all died until I finally saw the teeniest, tiniest new anemones. Then, I found more and more...
Re settlement - I was going off what I know for corals, what can be very specific per species; others just want a biofilm. I did do a quick and dirty scan of journals, but I didn't come up with anything. So, I gave them pretty much everything - clam shells, rocks that had been in the system, small gravel. In the end, they settled on pretty much anything - even the aquarium glass.
2nd question - in the 4th photo I see a lot of spheres. I assume that is a food source(?) What are you using - it doesn't look like decapsulated brine shrimp...
Yes, I was throwing whatever I could at them. Frozen rotifers, newly-hatched decapsulated brine, and yes, I did use decapsulated brine eggs. However, in that particular photo, the decapsulation method I was using for that batch of brine eggs didn't go so well (I was trying something new), so I went back to my old faithful after that. So, yeah, there were some brine eggs that never lost the outer cyst/shell.
Today they are big enough to eat whole frozen mysis shrimps.
BTW - thank you for sharing! Those are amazing photos! Are you conducting research?
Thanks. I wasn't intending to do any research with these - just display animals. I simply walked in one morning (actually, it was three different mornings for the two different species), and the tank was cloudy. However, instead of dumping everything down the drain with a water change, I gave it the 'ol college try - and it worked! But, far too many uncontrollables for a paper.
However, I did use the same fertilization and rearing techniques we use with coral larvae, so that may have helped my cause...????
Did you get any pic's of the spawning event?
MANY!
This is the first spawn, with the U. columbiana. I walked in and everything was pretty much over. Only a few eggs were floating around the tank, and one individual was still releasing sperm:
I got a turkey baster and siphoned as much sperm with as little water as possible and threw it under the scope:
Then, that painstaking process of harvesting eggs with a pipette from the water column:
I put egg and sperm together in a 50mL vial, but fertilization almost certainly happened in the water column - unless it was self-fert, which, I'm assuming only a genetic test will be able to identify...??? All told with the U. columbiana, I only collected about 50 eggs, and today there are a dozen left.
The U. crassicornis was a differentl story. I caught them in the act on two separate days!
Male repeasing sperm:
Eggs were just dribbling out of the females, and I didn't have all day - so I squeezed them...
And got this:
I then put them in 50mL vials for fert, matching basically what we do with coral egg/sperm. You try to make the water look about like "lemonade" for proper sperm concentration. But again... I cannot be sure that fertilization didn't happen in the tank already.
Interesting - the U. columbiana eggs were floating mid column - almost neutrally buoyant. The U. crassicornis eggs were definitely positively buoyant - look at all that cheddar on the surface!!!
Your third pic, taken "a few hours after spawning", shows what appears to be tentacle formation. This leads me to believe that fertilization did not take place after spawning. A few hours simply isn't enough time to go from an egg to an animals with tentacles. Development must have started inside the mother polyp. This is where that fine line comes in. larva that develop inside the animal may be produced sexually or asexually. We would need genetic testing, or necropsy of mother polyps to determine how the offspring are being produced.
That's the blastula phase - they turn into these, almost spiky balls. Gastrulation would have taken place after that, but I never got any pics - it must have happened over night b/c when I got in the next morning, they were back to being spherical. They floated for a few days before developing into this weird, "mushroom phase" that looks like one big ball sitting on top of a small ball. They swam around like that for several days, I'm assuming looking for a settlemnt cue. These guys also in the end mostly settled on the glass.
Here is a pic from our Singapore Coral Spawning Workshop - spawn collected from the surface slick with a potpourri of coral species, some which are "blastulating."
Perhaps this deserves its own thread...? I dunno...
Cheers
Mike