<a href=showthread.php?s=&postid=9457218#post9457218 target=_blank>Originally posted</a> by Huhhhhh
Good Job. Did you actually cut the shroom?:thumbsup:
Yeah, if you go to garf.org and look up trevors mushroom propagation you can see how i did it. Here is the abstract for my project.
The coral reefs are being destroyed, partially due to over collection. Fragment propagation of coral is a possible solution to this problem. Fragmenting wounds coral, necessitating its move to a quarantine aquarium. Quarantine is stressful to coral, often resulting in its sickness or death. There is need to expedite healing, and transfer fragments back to the main aquarium.
This project was designed to determine whether fragmented corallimorphs, genus Actinodiscus, would display faster healing with Ascorbic Acid supplementation. Six coralimorphs were fragmented. Four random fragments were placed in three substrate-filled Petri dishes, then placed in three aquariums. Two aquariums were respectively supplemented with 0.3mL and 0.6mL Ascorbic Acid. Control aquarium was left un-supplemented. Water was monitored for Nitrates, pH, KH, Specific Gravity, and Temperature. Healing was determined by measuring with a caliper, corallimorph oral disc diameter, every other day, over a 20-day period.
On day 10, the un-supplemented corallimorphs started to shrink, dying by day 14. The Ascorbic Acid supplemented corallimorphs continued thriving and their oral discs grew. Corallimorphs supplemented with 0.3mL Ascorbic Acid grew an average of 2.4mm, and 1.9mm respectively. Healthy corallimorphs were moved back to the main aquarium at 3 weeks.
Findings suggest that tissue regeneration in coral fragmenting occurs more readily with Ascorbic Acid supplementation. Addition of Ascorbic Acid to fragmented coral could make fragmenting a more successful option for aquarists and scientists, negating necessity to over collect from coral reefs.