Italy's Best! Matured SPS Aquariums.

[solbby]

continued...from another part of the paper.

Feeding might therefore have enhanced the construction of the organic matrix by (i) supplying additional input of energy, espercially for the dark processes. Under high plankton concentrations...........uptake of organic carbon (and hence energy) may be significant and could provide some energy for calcium/proton exchange at night. Alternatively, the larger biomass of fed corals may have provided larger energy stores for dark processes. Thus, feeding might have (ii) directly provided the necessary 'external' amino acids and/or (iii) indirectly increased photosynthesis and therefore the supply of 'autotrophic' amino acids.

 

[solbby]

So in conclusion from the thoughts above, we have a internally synthesized pool of amino acids created via photosynthesis and secondary metabolism and an amino acid that is naturally limited in coral tissue (no storage) but is also the most abundant within the coral organic matrix.

And this amino acid must be supplied externally. In nature this occurs via active feeding by corals.

 

[Herpervet]

It would be interesting to look at the amino acid profile of the shellfish diet the Italians are feeding.

L-Aspartic acid is a relatively inexpensive supplement. Was there any quantified data in any of these studies. i.e. how much of the aspartate was actually layed down? This would help with figuring out a dose.

 

[Sir]

<a href=showthread.php?s=&postid=8750403#post8750403 target=_blank>Originally posted</a> by Herpervet
It would be interesting to look at the amino acid profile of the shellfish diet the Italians are feeding.

L-Aspartic acid is a relatively inexpensive supplement. Was there any quantified data in any of these studies. i.e. how much of the aspartate was actually layed down? This would help with figuring out a dose.

L'acido aspartico è proprio uno di quegli amminoacidi che vorrei provare a dosare singolarmente, perchè correlato direttamente alla calcificazione....devo provare!sarei interessato anche io a capire le quantità che stai aggiungendo, solbby...grazie!

 

[Sir]

<a href=showthread.php?s=&postid=8748491#post8748491 target=_blank>Originally posted</a> by solbby
If the theory is that HGH is broken down to amino acids then why not use BSA? BSA = Bovine serum albumin. Much less expensive, highly concentrated in amino acids when degraded, readily available, and less wasteful from a human perspective, but I guess Italy is a rich country with lots of recombinant genetic labs at the aquarist's beckon call. But then again why use a protein at all, since pharmacies sells amino acids?

ciao! hai ragione..in effetti stò cercando qualcosa di meno costoso, ma comunque efficace per la crescita...come ti ho scritto nell'altro post, voglio provare l'acido aspartico...secondo me ha delel ottime potenzialità ed è poco costoso...Interessante anche la BSA, di cui mi parli...

 

[Robert Patterso]

Ok, I've read the entire post now, my brain feels like jello. So if I read this right, you combine everything together, mussel,oyster,shrimp,clam with the fructose and ro/di water and freeze it. Feed every three days. Or do you add the fructose to the tank 4 hrs before feeding then feed with the cubes. My plan would be to use a glutamine, which if I got this right is where the amino acids come from?

On another forum a guy made a mush of jarred/canned oysters and glutamine with very good result. Showed growth pics over a 30 day period.

I have a tank full of acros/sps which are growing ok, but would like to give this a 60-90 day run to see results.

 

[lead]

I want to start off by saying thanks to all of the information shared here. I have gone through this thread and read it several times. After reading this thread for hunredth time, I decided to try amino acid aproach since HGH is illegal here. So I went to the GNC and bought a very basic body building product. I used the basic shellfish recipe and added body building supplement. I fed my first cube on Tuesday. Well tonight, I saw the most amazing thing happen. I was sitting in my lazyboy chair by my tank and saw my two dersa clams start to spawn. This has never happened before in my tank. There has to be something to this. I have had SPS tanks for 5 years now and I have never had my clams spawn before.

 

[Sir]

<a href=showthread.php?s=&postid=8756005#post8756005 target=_blank>Originally posted</a> by lead
I want to start off by saying thanks to all of the information shared here. I have gone through this thread and read it several times. After reading this thread for hunredth time, I decided to try amino acid aproach since HGH is illegal here. So I went to the GNC and bought a very basic body building product. I used the basic shellfish recipe and added body building supplement. I fed my first cube on Tuesday. Well tonight, I saw the most amazing thing happen. I was sitting in my lazyboy chair by my tank and saw my two dersa clams start to spawn. This has never happened before in my tank. There has to be something to this. I have had SPS tanks for 5 years now and I have never had my clams spawn before.

Congratulazioni!!!!!!
probabilmente però sei tu che gestisci bene la vasca e questo ti ha portato ad ottenere questi risultati...per il pappone continua a darlo e vedrai che ti troverai bene!

ciao!!!

 

[Sir]

<a href=showthread.php?s=&postid=8754183#post8754183 target=_blank>Originally posted</a> by Robert Patterso
Ok, I've read the entire post now, my brain feels like jello. So if I read this right, you combine everything together, mussel,oyster,shrimp,clam with the fructose and ro/di water and freeze it. Feed every three days. Or do you add the fructose to the tank 4 hrs before feeding then feed with the cubes. My plan would be to use a glutamine, which if I got this right is where the amino acids come from?

On another forum a guy made a mush of jarred/canned oysters and glutamine with very good result. Showed growth pics over a 30 day period.

I have a tank full of acros/sps which are growing ok, but would like to give this a 60-90 day run to see results.

Robert, il saccarosio o fruttosio, dipende da quale preferisci, puoi metterlo direttamente nel pappone. Non serve dosarlo 4 ore prima...casomai puoi dosare 1-2 ore pirma del pappone, un pò di amminoacidi...va bene anche la glutammina.
ciao

 

[invincible569]

<a href=showthread.php?s=&postid=8749361#post8749361 target=_blank>Originally posted</a> by Henry Bowman
Invincible569

Where can I find the information on Zeovit that you referenced in your last post?

Thats an old thread. It was probably under the Captive Oceans forum which is no more. Maybe someone saved a link.

 

[invincible569]

<a href=showthread.php?s=&postid=8750109#post8750109 target=_blank>Originally posted</a> by solbby
Hi invincible!!! What would you want to test? specifically? .

I would love to see where the HGH magic happens which is during the freezing part of making the cubes. Is it possible for someone like you to obtain HGH and run lab tests on this procedure?

 

[lead]

Could someone translate the last few posting please. I would really apreciate it.
Thanks

 

[N-A-S-O]

<a href=showthread.php?s=&postid=8756005#post8756005 target=_blank>Originally posted</a> by lead
I want to start off by saying thanks to all of the information shared here. I have gone through this thread and read it several times. After reading this thread for hunredth time, I decided to try amino acid aproach since HGH is illegal here. So I went to the GNC and bought a very basic body building product. I used the basic shellfish recipe and added body building supplement. I fed my first cube on Tuesday. Well tonight, I saw the most amazing thing happen. I was sitting in my lazyboy chair by my tank and saw my two dersa clams start to spawn. This has never happened before in my tank. There has to be something to this. I have had SPS tanks for 5 years now and I have never had my clams spawn before.

"So I went to the GNC and bought a very basic body building product"............. and what would that be?????

 

[sprite]

Congratulazioni!!!!!!probabilmente per� six you that you manage the bathtub and this well has carried you to obtain these turns out to you... for the continuous glutton to give it and will see that you will find yourself well!


Robert, saccarosio or the fructose, depends from which you prefer, you can put it in the glutton directly. Servants not to dose it 4 hours before... casomai you can dose 1-2 hours pirma of the glutton, a p� of amino acids... goes also the glutammina well

 

[Aqua Keepers]

Ok the Italian to English translations still make no sence to me. Can someone break down what is going on in this thread?

 

[Robert Patterso]

I'm sorry, I just don't quite understand the translation.

Robert, saccarosio or the fructose, depends from which you prefer, you can put it in the glutton directly. Servants not to dose it 4 hours before... casomai you can dose 1-2 hours pirma of the glutton, a p� of amino acids... goes also the glutammina well

The fructose or sugar can be but into the glutton. Glutton meaning food or glutamine?

Casomai, meaning shell fish? Feed it 1-2 hrs prior to the glutamine or after the glutamine.

a p� of amino acids... goes also the glutammina well, does this mean that glutamine is a good source of amino acids?

Sorry, don't mean to be a pain here, just want to make sure I'm following this correctly.
This one heck of a thread with some great info. I appreciate all the guys from Italy sharing there experiences here...

 

[Leonardo_]

I think Glutton is a false translation (by translate-computer) for "pappone" wich is the blender-mush itself.

Leonardo

 

[Leonardo_]

Maybe it's time for a summary, so all the information of this whole thread is in one post. That post then can be checked by our Italian friends.

Just a thought...

Leonardo

 

[solbby]

<a href=showthread.php?s=&postid=8750403#post8750403 target=_blank>Originally posted</a> by Herpervet

L-Aspartic acid is a relatively inexpensive supplement. Was there any quantified data in any of these studies. i.e. how much of the aspartate was actually layed down? This would help with figuring out a dose.

Yes there was, but most of it is in another cited paper that works out the experimental design of using radioactive L-aspartic acid for coral uptake.

From the paper:
Organic matrix synthesis in the scleractinian coral stylophora pistillata: role in biomineralization and potential target of the organotin tributyltin

D Allemand, E TambuttE, JP Girard and J Jaubert
Journal of Experimental Biology, Vol 201, Issue 13 2001-2009, 1998.

Rates of incorporation of 14C-labelled macromolecules in to the organic matrix and calcification are constant over the time range tested, demonstrating that the ratio Ca2+/aspartic acid is kept constant and that both organic and inorganic skeletal components are depositied simultaneously.

(continued)............this allows us to calculate a rate of 0.13pmol aspartic acid h-1 mg-1 tissue protein incorporated in the skeleton, which is 4 x 10exp5 times slower than the rate of calcium deposition. This gives a molar ratio of Ca2+/aspartic acid of approximately 3.8 x 10exp6 .

Also from the Materials and Methods section:

Unless otherwise specified, the external concentration was adjusted to 0.5 mmol l-1 with unlabelled aspartic acid.

Therefore, approximation 1.9 mmol/US gallon would be the dose I would use.

 

[jmann]

The way I have read it you feed the amino's a few hrs before feeding the proteins/sugar 1/2 cube per 100 gals of tank size. So say you are using L-Glutamine you feed it a few hrs before the mush mix, is this correct???? Also maybe I'm getting old but what is sr 25 to 30??? John